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Catalog Number: (PI24600)

Supplier: Invitrogen

Description: Pierce Silver Stain for Mass Spectrometry is a complete kit for rapid and ultra-sensitive silver staining of proteins in polyacrylamide gels and efficient destaining of excised gel pieces for mass spectrometry analysis. This kit enables both first-time and experienced users to achieve consistent and reliable staining using high-, low-, and gradient-percentage gels in 1D and 2D formats. The optimized staining method ensures extremely sensitive staining while minimizing covalent crosslinking of protein to the gel matrix, which can inhibit protein recovery. The destaining reagents facilitate complete removal of silver from stained protein bands and maximum protein recovery for subsequent mass spectrometry analysis.

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Supplier: Invitrogen

Description: n-Dodecyl-β-D-Maltoside is especially useful for solubilizing membrane proteins to preserve their activity. Store below -20˚C.

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Supplier: Invitrogen

Description: Thermo Scientific NeutrAvidin coated plates are designed for binding biotinylated oligonucleotides and peptides with higher binding efficiency than other commercially available plates.

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Supplier: Invitrogen

Description: 50 mg

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Supplier: Invitrogen

Description: Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

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Supplier: Invitrogen

Description: Purified (unconjugated) Thermo Scientific Pierce™ Recombinant Protein G is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

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Supplier: Invitrogen

Description: The Thermo Scientific Pierce Gaussia-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Gaussia and Red Firefly luciferase activity in mammalian whole cell lysates.

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Supplier: Invitrogen

Description: <i>Gaussia</i> Luc vectors contain a gene cloned from the copepod, <i>Gaussia princeps</i>. The gene encodes a naturally-secreted bioluminescent <i>Gaussia</i> luciferase (approx. 20 kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates.

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Supplier: Invitrogen

Description: Thermo Scientific Halt Protease Inhibitor Cocktail (100X) are ready-to-use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction from animal and plant tissues, cultured cells,
yeast or bacteria.

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Supplier: Invitrogen

Description: Thermo Scientific Pierce Anti-c-Myc Magnetic Beads are affinity particles for immunoprecipitation of recombinant c-Myc-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.

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Supplier: Invitrogen

Description: Thermo Scientific SulfoLink Coupling Resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilization of cysteine-peptides and other sulfhydryl molecules.

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Catalog Number: (PI77120)

Supplier: Invitrogen

Description: Imject™ Ovalbumin is a purified ovalbumin (OVA) protein conveniently formulated for preparation of hapten-carrier conjugates.

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Supplier: Invitrogen

Description: The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

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Catalog Number: (PI26181)

Supplier: Invitrogen

Description: 26181 is supplied as 2 ml of antibody on crosslinked 4% beaded agarose supplied as a 50% slurry in 0. 1M phosphate, 0. 15M NaCl, pH 7. 2 with 0. 05% sodium azide. Specific for the HA peptide YPYDVPDYA. The anti-HA agarose has been used successfully in immunoprecipitation applications. The anti-HA agarose has 3. 5mg mouse anti-HA IgG1 monoclonal antibody conjugated per mL of settled agarose resin. HA Synthetic Peptide is available for use in neutralization and control experiments and to competitively elute HA-tagged fusion proteins from immobilized anti-HA affinity resin. An HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein when doing studies with transfected cells.

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Supplier: Invitrogen

Description: Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications

PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution, and a complete kit containing substrate solution and diethanolamine buffer.

Thermo Scientific Pierce PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.

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Supplier: Invitrogen

Description: PCC-54 Detergent Concentrate is a general cleaning agent for use on many laboratory surface materials and glassware, including lenses, mirrors, and reflectors.

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