You Searched For: Electrophoresis Buffers

Description: This antimycotic interferes with fungal membrane permeability by forming channels in cell membranes, causing small molecules to leak from the cell. It is recommended for use in cell culture applications at 10 ml/L and is stable at 37 ºC for 3 days.

Catalog Number: 45000-808

Supplier: Corning

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Description: Hanks' Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological pH for cells maintained in non-CO₂ atmospheric conditions.

Catalog Number: 45000-464

Supplier: Corning

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Description: Miller's LB broth is based on the original Lysogeny Broth (LB) media formulation developed by Luria and Bertani, which was designed for the rapid propagation of recombinant <i>E. coli</i> strains.

Catalog Number: 45001-048

Supplier: Corning

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Description: Dimethyl Sulfoxide (DMSO) is commonly used in cell cryopreservation, along with glycerol, to prevent damage to the cell membrane during freezing.

Catalog Number: 45001-118

Supplier: Corning

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Description: Trypan Blue is the most common stain used in cell count and viability assays.

Catalog Number: 45000-717

Supplier: Corning

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Description: The addition of MEM nonessential amino acids to medium can improve growth and viability of propagating cells in culture while reducing the biosynthetic burden on these cells

Catalog Number: 45000-700

Supplier: Corning

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Description: EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+.  These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity.  The use of EDTA therefore allows for greater control over many experiments.

Catalog Number: 45001-122

Supplier: Corning

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Description: Molecular biology grade water is ideal for a wide variety of routine tasks requiring a high degree of sterility Molecular biology grade water is ideal for the preparation of reagents, rinsing glassware and plasticware, and other molecular biology applications where RNase-, DNase-, and Protease-free water is required. No toxic agents, including DEPC, are used in the preparation of Mediatech's molecular biology grade water, eliminating possible interferences with enzymatic reactions.

Catalog Number: 95000-094

Supplier: Corning

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Description: 0,05% Trypsin/0,53 mM EDTA in HBSS (Hank’s Balanced Salt Solution) with sodium bicarbonate, without calcium and magnesium.

Catalog Number: 45001-082

Supplier: Corning

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Description: L-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions, producing toxic compounds. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use. This formulation is prepared in cell culture grade water.

Catalog Number: 45000-676

Supplier: Corning

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Description: HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2

Catalog Number: 45000-690

Supplier: Corning

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Description: A modification of Ham's F-12, F-12K contains higher concentrations of amino acids, sodium pyruvate and other components than the original

Catalog Number: 45000-354

Supplier: Corning

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Description: This is a combination of penicillin, streptomycin and Amphotericin formulated to contain 10000 units/ml penicillin G, 10 mg/ml streptomycin sulfate and 25 µg/ml amphotericin B.

Catalog Number: 45000-616

Supplier: Corning

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Description: Terrific Broth is a medium for the propagation and maintenance of recombinant E.coli.

Catalog Number: 45001-050

Supplier: Corning

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Description: 45% Glucose Solution. Glucose is a main source of energy for living organisms and is used as a nutrient source in cell culture media. This 45% (w/v) solution has been to ensure no cytotoxicity.

Catalog Number: 45001-116

Supplier: Corning

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Description: Named for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy's 5A containing an increased level of inositol.

Catalog Number: 45000-410

Supplier: Corning

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