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Supplier: MP Biomedicals

Description: Storage: Room Temperature
2-Mercaptoethanol is a hybrid of ethylene glycol and 1,2-ethanedithiol. It is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. It is widely used because the hydroxyl group confers solubility in water and lowers the volatility. Due to its diminished vapor pressure, its odour, while unpleasant, is less objectionable than related thiols.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also it reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

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Supplier: MP Biomedicals

Description: Storage: Store at Room Temperature (15 - 30 °C)
Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component. Glycerol is used in the concentration and storage of enzymes. Also prevents back-diffusion and protein samples into the buffer. Ideal for use as a cryoprotectant.

Supplier: MP Biomedicals

Description: amino acid found in human plasma and thyroid gland, similar to thyroxine

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Supplier: MP Biomedicals

Description: Aprotinin is found in bovine lymph nodes, lung, parotid gland, spleen, liver, pancreas, seminal vesicles, thyroid gland, kidney, mucous membranes of the trachea and esophagus, ovaries, heart, posterior pituitary and cartilage. It is a serine protease inhibitor which is reactive against trypsin, chymotrypsin, plasmatic and glandular kininogenases, plasmin, kallkrein, urokinase, clotting factor XIIa, protein C, proteinases of the complement system, leukocyte and tissue proteinases. It does not inhibit thrombin. Aprotinin works by blocking the active sites of enzymes. Binding is reversible with most aprotinin-protease complexes dissociating at pH > 10 or < 3.

Supplier: MP Biomedicals

Description: Digitonin is a mild nonionic detergent. It permeabilizes plasma membranes of eukaryotic cells by complexing with membrane cholesterol and other unconjugated β-hydroxysterols.

Catalog Number: (IC0219390010)

Supplier: MP Biomedicals

Description: Methods for immobilizing insulin on polystyrene dishes have been reported for applications involving protein-free cell cultures.

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Supplier: MP Biomedicals

Description: Easily soluble in diethyl ether; soluble in acetone,chloroform; slightly soluble in alcohol.

SDS

Supplier: MP Biomedicals

Description: Spermidine is a biogenic polyamine formed from putrescine. It is essential in both normal and neoplastic tissue growth. It plays an important role in the regulation of cellular proliferation and differentiation. Forms stable compounds with nucleic acids. Inhibits neuronal nitric oxide synthase (nNOS).

SDS

Supplier: MP Biomedicals

Description: Penicillin G is a narrow spectrum antibiotic

SDS

Supplier: MP Biomedicals

Description: Adenosine 5′-monophosphate is a nucleotide that is used as a monomer in RNA. It is an ester of phosphoric acid and the nucleoside adenosine. AMP consists of a phosphate group, the sugar ribose, and the nucleobase adenine.

SDS

Supplier: MP Biomedicals

Description: α - Naphthyl phosphate disodium salt is a substrate for determination of phosphatase.

SDS

Supplier: MP Biomedicals

Description: 5-Bromo-2’-deoxyuridine is a thymidine analog and useful for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. It can be used to label DNA which can be used in conjunction with BrdU Monoclonal Antibody for the immunohistochemical analysis of cell proliferation.

SDS

Supplier: MP Biomedicals

Description: Squalene, an isoprenoid compound structurally similar to beta-carotene, is an intermediate metabolite in the synthesis of cholesterol.

SDS

Supplier: MP Biomedicals

Description: Thiamine (Vitamin B1) is one of the essential vitamins and is required for carbohydrate metabolism.

SDS

Supplier: MP Biomedicals

Description: Guanosine-5'-monophosphate is an ester of phosphoric acid with the nucleoside guanosine.

SDS

Supplier: MP Biomedicals

Description: Hydrocortisone, a primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na2+ retention potency. Hydrocortisone, known more formally as cortisone (INN, USAN, BAN), is a steroid hormone, produced by the zona fasciculata of the adrenal gland. It is released in response to stress and a low level of blood glucocorticoids.

SDS