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Catalog Number: (IC091676549)
Supplier: MP Biomedicals
Description: MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media.


Catalog Number: (75877-480)
Supplier: MP Biomedicals
Description: Fluorescein-conjugated Goat IgG fraction to Rabbit IgG is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to rabbit IgG (whole molecule) and buffer salts.

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Anti-Rabbit IgG (whole molecule)-FITC antibody reacts with rabbit serum and rabbit IgG.

Fluorescein-conjugated Goat IgG fraction to Rabbit IgG is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).

Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.

The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The IgG fraction is tested for purity and specificity at 40 mg/mL using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected.


Catalog Number: (IC3050044)
Supplier: MP Biomedicals
Description: Mycoplasma removal agent (MRA) is a highly effective solution for the serious problem of mycoplasma contamination in cell cultures.

SDS


Catalog Number: (IC114550422)
Supplier: MP Biomedicals
Description: Double dropout formulation of synthetic defined (SD) yeast media which includes all necessary components for selective growth of <i>S. cerevisiae</i>. SD and SD with agar (SDA) in a variety of formats for flexibility and convenience.


Catalog Number: (IC0856007)
Supplier: MP Biomedicals
Description: Product is the lyophilized powder of goat antiserum to swine serum and buffer salts.


Catalog Number: (IC0855111)
Supplier: MP Biomedicals
Description: Product is liquid goat antiserum to human alpha-1-antitrypsin and buffer salts.
The antiserum is prepared by hyperimmunizing goats with highly purified human alpha-1-antitrypsin; antiserum is delipidated
and made specific by solid-phase absorption. The resulting antiserum is dialyzed into 0.02M sodium phosphate, 0.14M sodium
chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed.

Catalog Number: (IC116850200)
Supplier: MP Biomedicals
Description: BioPulverizer™ System II contains 10x2 mL tubes of each of our specialized Lysing Matrices.


Catalog Number: (IC091682049)
Supplier: MP Biomedicals
Description: Sodium pyruvate is used by cells as an easily accessible carbohydrate source. Additionally, it is involved with amino acid metabolism and initiates the Kreb′s cycle. The 100 mM solution should be diluted 1:100 for most cell culture. The use of sodium pyruvate in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid by Bacillus sphaericus has been described. A protocol that uses sodium pyruvate to establish stably transfected human B cell lines has been published. It improves coliform recovery when present in culture medium.


Supplier: MP Biomedicals
Description: Shikimic acid, more commonly known as its anionic form shikimate, is an important biochemical metabolite in plants and microorganisms. Shikimic acid is a precursor for the aromatic amino acids phenylalanine and tyrosine, indole, indole derivatives and aromatic amino acid tryptophan, many alkaloids and other aromatic metabolites, phenylpropanoids, flavonoids, tannins, and lignin.

SDS

Supplier: MP Biomedicals
Description: Vitamin B12 plays a role in the synthesis of myelin. It is a cofactor for two enzymes, methionine synthase and L-methylmalonyl-CoA mutase. The compound is also used as an antipernicious anemia factor.

SDS

Catalog Number: (IC15588125)
Supplier: MP Biomedicals
Description: Nitrofurantoin is an antibactericidal compound that has been historically prepared by the reaction of 1-aminohydantoin sulphate and 5-nitro-2-furaldehyde diacetate.

SDS


Supplier: MP Biomedicals
Description: Demecolcine, also known as colcemid, is a drug used in chemotherapy. It is closely related to the natural alkaloid colchicine with the replacement of the acetyl group on the amino moiety with methyl, but it is less toxic.
Demecolcine is an antineoplastic agent used to immobilize chromosomes by inactivating the spindle fiber mechanism during metaphase. Medically, Demecolcine has been used to improve the results of cancer radiotherapy by synchronising tumour cells at metaphase, the radiosensitive stage of the cell cycle.
Demecolcine depolymerizes microtubules; blocks mitosis at metaphase. Often in karyotyping and cell cycle research it is desirable to increase the yield of mitotic cells in a particular phase of the cell cycle. This can be achieved in a variety of ways with the most popular being the use of a cell cycle synchronizing agent such as demecolcine. Demecolcine will arrest cells in metaphase with no remarkable effect on the biochemical events in mitotic cells or in synchronized G1 and S phase cells. White blood cells are often treated with demecolcine to arrest cells in metaphase.

SDS

Supplier: MP Biomedicals
Description: Freely soluble in ether and glycerol; soluble in alcohol(111 mg/mL), water (50mg/mL -clear, colorless solution); slightly soluble in chloroform.

SDS

Supplier: MP Biomedicals
Description: Glutamate is a key compound in cellular metabolism. It is the most abundant excitatory neurotransmitter in the vertebrate nervous system. It also serves as the precursor for the synthesis of the inhibitory GABA in GABA-ergic neurons. This reaction is catalyzed by glutamate decarboxylase (GAD), which is most abundant in the cerebellum and pancreas.
Supplier: MP Biomedicals
Description: ~28 IU/mg, Powder

Supplier: MP Biomedicals
Description: 5-Bromo-2'-deoxycytidine can be incorporated into DNA in the presence of DNA polymerase, replacing dCTP.

SDS

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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