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Description: The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002; Chong et al., 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser301 and Ser642.
Catalog Number: 10075-444
Supplier: Prosci


Description: Phospho-Raf1 (S301) ELISA Kit
Catalog Number: ABCA_AB279903-1X96
Supplier: ABCAM INC.

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Description: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.
Catalog Number: 10802-250
Supplier: Rockland Immunochemical


Description: Simple Western
Catalog Number: 220011-097
Supplier: R&D Systems


Description: Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Catalog Number: 10350-006
Supplier: Bioss


Catalog Number: 89395-242
Supplier: Abgent


Description: Rabbit polyclonal antibody to C-RAF for WB, IHC and ELISA with samples derived from Human, Mouse and Rat.
Catalog Number: 77052-152
Supplier: ANTIBODIES.COM LLC


Description: Rabbit polyclonal antibody to C-RAF for WB and ELISA with samples derived from Human, Mouse and Rat.
Catalog Number: 77058-982
Supplier: ANTIBODIES.COM LLC


Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of RAF1.
Catalog Number: 10680-702
Supplier: Abnova


Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of RAF1.
Catalog Number: 10680-708
Supplier: Abnova


Description: Rabbit polyclonal antibody to C-RAF for WB, IHC and ELISA with samples derived from Human, Mouse and Rat.
Catalog Number: 77052-148
Supplier: ANTIBODIES.COM LLC


Description: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
Catalog Number: 10813-724
Supplier: Prosci


Description: Rabbit polyclonal antibody raised against synthetic peptide of BRAF.
Catalog Number: 10678-316
Supplier: Abnova


Catalog Number: 89395-230
Supplier: Abgent


Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of RAF1.
Catalog Number: 10680-706
Supplier: Abnova


Description: Rabbit Polyclonal antibody to GABA B Receptor 2 (gamma-aminobutyric acid (GABA) B receptor, 2)
Catalog Number: 89319-518
Supplier: Genetex


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