You Searched For: OPTEM

Catalog Number: (103260-182)

Supplier: Southern Biotechnology

Description: CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80% of normal peripheral blood lymphocytes and on 10-20% of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation. The monoclonal antibody UCHT1 reacts with the 20 kDa ε chain of the CD3/TCR complex.

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Catalog Number: (100245-026)

Supplier: Southern Biotechnology

Description: Goat Anti-Canine IgG(H+L)-AP antibody for use in ELISA, immunohistochemistry / immunocytochemistry, and western blotting assays.

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Catalog Number: (103260-020)

Supplier: Southern Biotechnology

Description: Goat Anti-Human Lambda-FITC antibody with minimal reactivity to mouse proteins for use in flow cytometry assays.

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Catalog Number: (100244-292)

Supplier: Southern Biotechnology

Description: Goat F(ab')2 Anti-Human IgG-AP antibody for use in ELISA, immunohistochemistry, western blotting, and ELISpot assays.

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Catalog Number: (103265-110)

Supplier: Southern Biotechnology

Description: Donkey F(ab')2 Anti-Rabbit IgG(H+L)-Alexa Fluor 647 antibody with minimal reactivity to human, mouse, rat, bovine, horse, hamster, goat, sheep, chicken, and guinea pig serum proteins for use in fluorescent assays.

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Catalog Number: (103265-620)

Supplier: Southern Biotechnology

Description: The CD117 (c-Kit) antigen is the cell-surface receptor for stem cell factor (SCF). Together these molecules constitute a ligand/receptor pair which functions to maintain normal hematopoiesis in the adult. Signaling through SCF/c-Kit has an important role in stimulating myeloid and erythroid production of primitive hematopoietic progenitor cells. A variety of cytokines act synergistically with SCF to stimulate proliferation and differentiation of bone marrow progenitor cells. For example, SCF plus IL-7 can stimulate the combined myeloid and B cell differentiation of uncommitted hematopoietic progenitor cells. SCF/c-Kit also participates in erythropoiesis in both the bone marrow and spleen. Loss-of-function mutations for SCF/c-Kit lead to a variety of pleiotropic developmental defects while gain-of-function mutations can lead to constitutive activation of the kit receptor and links to cancer. Examples of the former are mast cell deficiency and severe macrocytic anemia while the latter mutation has been identified in mastocytomas.

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Catalog Number: (100243-270)

Supplier: Southern Biotechnology

Description: CD19 is a monomeric transmembrane glycoprotein expressed at relatively constant levels throughout B cell development from early pro-B/pre-B cells (i.e. B220+/CD43+/HSA+) through fully differentiated B cell stages. Terminally differentiated plasma cells do not express CD19. In humans, the CD19 molecule on the surface of mature B cells associates with CD21 (CR-2) and CD81 (TAPA-1), and this multimolecular complex synergizes with surface immunoglobulin to provide signal transduction and promote cellular activation. All splenic and peritoneal IgM+ cells of both B-1 and B-2 lineages are CD19+, with B-1 cells expressing higher levels of CD19 than B-2 cells in these sites. Studies with CD19-deficient mice have suggested that this molecule may not be required for normal generation and maturation of B cells in the bone marrow. The monoclonal antibody 6D5 recognizes the same epitope as the published clone, 1D3.

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Catalog Number: (100243-742)

Supplier: Southern Biotechnology

Description: The immunoglobulin heavy chain binding protein BiP is a member of the hsp70 family of heat shock proteins and is identical to the glucose regulated protein GRP78. While BiP was originally described for its function in B cells, it is now known to be distributed in a variety of tissues, if not ubiquitous. The highly conserved hsp70 proteins have an essential physiological role in stress responses and as “molecular chaperones” which are responsible for a variety of functions such as protein transport, prevention of protein toxicity and direction of protein folding. With regard to its immunological role, BiP is a component of the endoplasmic reticulum and binds free intracellular heavy chains in nonsecreting pre-B cell lines (μ+,L-) or incompletely assembled Ig precursors in H+L+ secreting hybridomas and myelomas. In the absence of light chain synthesis, heavy chains remain associated with BiP and are not secreted. BiP is an ATP binding protein and the dissociation of the BiP-heavy chain complex is probably driven by the ATPase activity attributed to BiP. The monoclonal antibody 76-E6 recognizes a conserved epitope localized within the region of amino acids 497 to 581 of BiP.

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Catalog Number: (101190-302)

Supplier: Southern Biotechnology

Description: In the mouse, CD8 exists in two forms – (i) a CD8 heterodimer composed of an α chain (CD8α) and a β chain (CD8β); and (ii) a homodimer of two α chains. The heterodimer is found on the surface of essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8α without CD8β. It has been suggested that CD8+β- T cells mature extrathymically, while development of the CD8α+β+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8α+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

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Catalog Number: (103265-248)

Supplier: Southern Biotechnology

Description: CD49b, a member of the integrin family of extracellular matrix and cell adhesion molecules, is the integrin α2 chain. CD49b associates noncovalently with CD29, the integrin β1 subunit, to form the heterodimer integrin α2β1 that binds laminin and collagen. It is expressed on B and T lymphocytes, NK cells, monocytes, platelets, fibroblasts, and long-term cultured T cells. CD49b/CD29 mediates the Mg++-dependent adhesion of platelets to collagen. It may also be utilized by fibroblasts for the reorganization of the collagen matrix during wound healing.

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Catalog Number: (100245-530)

Supplier: Southern Biotechnology

Description: Biotin Anti-Chicken IgA antibody for use in flow cytometry, immunohistochemistry / immunocytochemistry, ELISA, and immunoprecipitation assays.

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Catalog Number: (101442-560)

Supplier: Southern Biotechnology

Description: Purified Rat IgG2b for use as a standard in ELISA.

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Catalog Number: (100245-414)

Supplier: Southern Biotechnology

Description: CD3 is a member of the T cell receptor-associated CD3 complex. The monoclonal antibody CT-3 recognizes a complex of at least three polypeptides of Mr 20, 19, and 17 kDa (two of which are N-glycosylated) on chicken T cells. The antibody also coprecipitates a polypeptide of 90 kDa from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of Mr 50 and 40 kDa.

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Catalog Number: (103260-120)

Supplier: Southern Biotechnology

Description: Anti-Biotin may be used for indirect immunostaining when working with a biotinylated primary antibody. As an effective alternative to avidin, Anti-Biotin amplifies signal and reduces background that can be caused by avidin and its derivatives.

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Catalog Number: (100243-886)

Supplier: Southern Biotechnology

Description: The monoclonal antibody 2F1 reacts with mouse killer cell lectin-like receptor G1 (KLRG1; formerly known as mouse MAFA), a homodimeric member of the lectin-like type 2 transmembrane receptor family that contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. The 2F1 antibody stains 30-60% of NK1.1+CD3- splenocytes, and a small fraction of T cells in all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL). Cell surface expression of KLRG1 is up-regulated by expression of MHC class I molecules. The effect of MHC class I expression is indirect, and can be mediated by interactions with class I-specific Ly49 inhibitory receptors.

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Catalog Number: (103264-642)

Supplier: Southern Biotechnology

Description: The CD117 (c-Kit) antigen is the cell-surface receptor for stem cell factor (SCF). Together these molecules constitute a ligand/receptor pair which functions to maintain normal hematopoiesis in the adult. Signaling through SCF/c-Kit has an important role in stimulating myeloid and erythroid production of primitive hematopoietic progenitor cells. A variety of cytokines act synergistically with SCF to stimulate proliferation and differentiation of bone marrow progenitor cells. For example, SCF plus IL-7 can stimulate the combined myeloid and B cell differentiation of uncommitted hematopoietic progenitor cells. SCF/c-Kit also participates in erythropoiesis in both the bone marrow and spleen. Loss-of-function mutations for SCF/c-Kit lead to a variety of pleiotropic developmental defects while gain-of-function mutations can lead to constitutive activation of the kit receptor and links to cancer. Examples of the former are mast cell deficiency and severe macrocytic anemia while the latter mutation has been identified in mastocytomas.

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