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Supplier: Biotium

Description: Cytochrome c is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic Cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, Cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of Cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of Cytochrome c and to induce cell death. The release of Cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a Cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total Cytochrome c which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

CF® dyes are Biotium's next-generation fluorescent dyes. CF®568 is a red fluorescent dye (Ex/Em 562/583 nm) with superior brightness and photostability. It also is compatible with super-resolution imaging by STORM and TIRF.

Supplier: Biotium

Description: This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®647 is a far-red fluorescent dye (Ex/Em 650/665 nm) with excellent brightness. It also is compatible with super-resolution imaging by STORM.

Supplier: Biotium

Description: This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®594 is a deep red fluorescent dye (Ex/Em 593/614 nm). It yields the brightest conjugates among spectrally similar dyes, and has excellent photostability.

Supplier: Biotium

Description: CellBrite™ Fix dyes are fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. They are unique among membrane stains in which they can withstand both fixation and detergent permeabilisation.

Supplier: Biotium

Description: This MAb is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81 kDa) and PR-B (116 kDa). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®405S is a blue fluorescent dye (Ex/Em 404/431 nm) with superior brightness compared to other blue dyes; it is also compatible with super-resolution imaging by SIM. Note: Conjugates of blue fluorescent dyes are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Supplier: Biotium

Description: CD56 / NCAM Monoclonal antibody, Clone: 123C3.D5, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: purified, Immunogen: Membrane preparation of a small cell lung carcinoma, Synonyms: NCAM, Leu-19, NKH1, Size: 100uL

Supplier: Biotium

Description: Cytokeratin 10 Monoclonal antibody, Clone: LH2, Host: Mouse, Species reactivity: Mouse, Human, Isotype: IgG2a, kappa, Conjugate: CF405S, Immunogen: Skin extract of a human Psoriasis patient, Synonyms: BCIE, BIE, EHK, Application: IF, IHC, FC, Size: 100uL

Supplier: Biotium

Description: CD66 Monoclonal antibody, Clone: CEA31, Host: Mouse, Species reactivity: Human, Monkey, Isotype: IgG1, kappa, Conjugate: purified, Immunogen: Human colon carcinoma extract, Synonyms: CEACAM5, Application: IF, IHC, Size: 500 uL

Catalog Number: (89493-538)

Supplier: Biotium

Description: Anti-IgG Goat Polyclonal Antibody (CF™ 514)

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Supplier: Biotium

Description: Kappa Light Chain, Monoclonal antibody, Clone: HP6053 + L1C1, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: CF488A, Immunogen: Purified human Ig kappa chain (HP6053) & Human B-Lymphoma Cells (L1C1), Synonyms: HCAK1, Size: 500uL

Supplier: Biotium

Description: NIR CF® dye free acids are non-reactive molecules and cannot be used for labeling. They can be used as reference standards or controls for corresponding NIR CF® dye-conjugates such as antibodies, proteins, peptides etc. in cell-based and in vivo assays.

Supplier: Biotium

Description: In Western blotting, this antibody detects an antigen of 125 kDa in human liver and 135 kDa in tumors of histiocytic origin. Comparative study of this MAb and a standard CD68 MAb showed that their antigens are different. Its antigen in all macrophage types studied is located on the plasma membrane and within cytoplasmic structures including lysosomes. This MAb shows a restricted reactivity to cells of the monocyte/macrophage system. It specifically reacts with blood monocytes and stains resident macrophages in a wide variety of human tissues. This MAb does not stain antigen-presenting cells, e.g., Langerhans cells. Reportedly, its reactivity is restricted to histiocytes and macrophages.

Supplier: Biotium

Description: CD66 Monoclonal antibody, Clone: COL-1, Host: Mouse, Species reactivity: Human, Isotype: IgG2a, kappa, Conjugate: CF647, Immunogen: Human colon carcinoma extract, Synonyms: CEACAM5, Application: ImmunoFluorescence, IHC, Size: 100uL

Supplier: Biotium

Description: TAG-72 / CA72.4, Monoclonal antibody, Clone: B72.3, Host: Mouse, Species reactivity: Human, Hamster, Dog, Cow, Rat, Isotype: IgG1, kappa, Conjugate: CF594, Immunogen: Membrane-enriched fraction of breast carcinoma, Synonyms: CA72.4, Size: 500uL

Supplier: Biotium

Description: This MAb is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81 kDa) and PR-B (116 kDa). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone. It is excellent for immunohistochemical staining of formalin/paraffin tissues.

CF® dyes are Biotium's next-generation fluorescent dyes. CF®640R is a far-red fluorescent dye (Ex/Em 642/662 nm) with excellent brightness, and the best photostabiity among spectrally-similar dyes.

Supplier: Biotium

Description: Cytokeratin 18 Monoclonal antibody, Clone: DA7, Host: Mouse, Species reactivity: Human, Isotype: IgG1, kappa, Conjugate: CF488A, Immunogen: Human breast cancer PMC 42 cells, Synonyms: K18, Application: IF, IHC, WB, FC, Size: 100uL