You Searched For: protein purification system

Supplier: Invitrogen

Description: Thermo Scientific Pierce Anti-c-Myc Agarose is a high-affinity immunoprecipitation resin ideal for purification of recombinant c-Myc-tagged proteins expressed in human <i>in vitro</i> expression systems and bacterial and mammalian cell lysates.

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Supplier: GE Healthcare - Life Sciences

Description: HiTrap™ Protein G HP affinity columns are pre-packed with 1 or 5 ml of Protein G Sepharose™ high performance for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.

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Supplier: GE Healthcare - Life Sciences

Description: GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.

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Catalog Number: (IB07062)

Supplier: IBI Scientific

Description: SDS is an ionic detergent used to denature proteins in hybridization, nucleic purification, and electrophoresis buffer systems. SDS is also used to dissociate nucleic acid protein complexes in DNA extraction protocols, to disrupt cell membranes and to prepare prehybridization and/or hybridization solutions.

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Supplier: Invitrogen

Description: The Thermo Scientific Pierce IgM Purification Kit is an easy-to-use affinity matrix and buffer system to purify IgM from mouse ascites fluid; the method uses immobilized mannan binding protein, which binds to IgM in the kit-supplied buffers.

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Catalog Number: (10273-218)

Supplier: Proteintech

Description: The VSX2 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human VSX2. This antibody recognizes human, mouse antigen. The VSX2 antibody has been validated for the following applications: ELISA, WB analysis.

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Supplier: MP Biomedicals

Description: FastPrep® non-skirted empty tubes are used to hold samples for cell lysis and homogenization.

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Catalog Number: (89361-078)

Supplier: Genetex

Description: Thioredoxin was found in yeast, plants, mammals and bacteria. It is a protein with two redox-active half-cystine residues in exposed active center, having the following amino acid sequence: -Cys-Gly-Pro-Cys. The protein exists in reduced form (Trx-(SH)2) with a dithiol, or in an oxidized form (Trx-S2). The ThioFusion Expression System allows expression and purification of large amounts of heterologous protein from E. coli.

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Catalog Number: (103010-690)

Supplier: Anaspec Inc

Description: This is biotinylated Protein A conjugate suitable for asssay development, IHC, IF, immunoprecipitation or western blotting.
AnaSpec's recombinant Protein A consists of only IgG binding domains and was expressed using a recombinant bacterial expression system. Its apparent molecular weight is approximately 30,000 Da compared to 42,000 Da for the native Protein A that is found in Staphylococcus aureus.
Protein A conjugates are widely used as labeled reagents for a variety of experiments including immunoprecipitation, antibody detection and purification and assay development

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Catalog Number: (77437-352)

Supplier: Bioss

Description: Epitope tagging is a recombinant DNA method by which a protein encoded by a cloned gene is made immunoreactive to a known antibody. Major areas ofapplication include monitoring protein expression, localizing proteins at thecellular and subcellular levels, and protein purification, as well as the analysis of protein topology, dynamics and interactions. Due to their small size, theyare unlikely to affect the tagged protein's biochemical properties.S1 tag is an epitope tag composed of a nine-residue peptide, NANNPDWDF, derived from the hepatitis B virus preS1 region. Epitope tags consisting ofshort sequences recognized by well-characterizated antibodies have beenwidely used in the study of protein expression in various systems.

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Catalog Number: (103010-688)

Supplier: Anaspec Inc

Description: AnaSpec's recombinant Protein A consists of only IgG binding domains and was expressed using a recombinant bacterial expression system. Its apparent molecular weight is approximately 30,000 Da compared to 42,000 Da for the native Protein A that is found in Staphylococcus aureus.
Protein A is a non-glycosylated cell wall protein of Staphylococcus aureus that can bind the Fc part of immunoglobulin molecule of different species with strong affinity (1-4). Protein A consists of five IgG binding domains (A, B, C, D, and E), each approximately 60 amino acids long with no cysteines present and two S. aureus cell membrane binding domains, X and M (3).
Application: Recombinant Protein A can be used for immunoprecipitation, antibodies purification, and assay development.

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Catalog Number: (77437-470)

Supplier: Bioss

Description: The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.

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Supplier: MP Biomedicals

Description: CellMaxx™ Bovine Albumin is chromatographically purified from New Zealand-sourced bovine plasma in an ISO quality system assuring complete traceability and consistent high quality. This albumin has very low endotoxin and low IgG to simplify downstream purification, is tested for the absence of viruses according to 9CFR and has the highest possible biosafety level. The albumin supports growth of stem cells and increases protein production from hybridoma cells.

Catalog Number: (103010-694)

Supplier: Anaspec Inc

Description: Protein A-HiLyte™ Fluor 555 Conjugate can be used as a universal reagent to detect primary antibodies in IHC from many species including rabbit, human, some mouse IgG isotypes, and others. Fluorescence (orange) Excitation/Emission wavelength: 553 nm/568 nm.
AnaSpec's recombinant Protein A consists of only IgG binding domains and was expressed using a recombinant bacterial expression system. Its apparent molecular weight is approximately 30,000 Da compared to 42,000 Da for the native Protein A that is found in Staphylococcus aureus.
Protein A conjugates are widely used as labeled reagents for a variety of experiments including immunoprecipitation, antibody detection and purification and assay development

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Catalog Number: (103010-692)

Supplier: Anaspec Inc

Description: Protein A-HiLyte™ Fluor 488 Conjugate can be used as a universal reagent to detect primary antibodies in IHC from many species including rabbit, human, some mouse IgG isotypes, and others. Fluorescence (green) Excitation/Emission wavelength: 499 nm/523 nm.
AnaSpec's recombinant Protein A consists of only IgG binding domains and was expressed using a recombinant bacterial expression system. Its apparent molecular weight is approximately 30,000 Da compared to 42,000 Da for the native Protein A that is found in Staphylococcus aureus.
Protein A conjugates are widely used as labeled reagents for a variety of experiments including immunoprecipitation, antibody detection and purification and assay development

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Catalog Number: (77007-608)

Supplier: Genscript

Description: Human IgG is the major immunoglobulin in blood and plays a key role in the humoral immune response. The Fc portion of human IgG binds to Fc receptor that can activate the complement system. Fc-tagged proteins are generated with DNA recombinant technology. The protein of interest is fused with the Fc tag. Most vectors encoding for Fc tag sequence use the Fc region of human IgG1. Protein A can be used for the purification of Fc-tagged proteins. Human IgG (Fc), mAb, Mouse is a useful tool for immunoassay of human Fc-tagged proteins and human IgG.GenScript Human IgG (Fc) Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with human Fc-tagged protein.

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