You Searched For: Antibodies

Catalog Number: (10799-982)

Supplier: Rockland Immunochemical

Description: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.

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Catalog Number: (RL611-7602)

Supplier: Rockland Immunochemical

Description: Secondary Donkey Anti-IgG (H&L) Reacts with Rabbit (Lapine)

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Catalog Number: (10049-828)

Supplier: Enzo Life Sciences

Description: Monoclonal Antibody, Primary Antibody, Used in ICC, IHC with species reactivity to Mouse, Rat, Host: Mouse, Immunogen: Recombinant rat agrin (C-terminal fragment), Isotype: IgG1

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Catalog Number: (89154-476)

Supplier: Enzo Life Sciences

Description: Host: Rat, Isotype: IgG2a

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Catalog Number: (10150-026)

Supplier: Immunoreagents

Description: ImmunoReagents’ Biotinylated antibody conjugates are widely used due to their ability to enhance antibody signal in most immunoassays. Biotinylated secondary antibodies are used in most immunoassay formats (i.e., ELISA, Immunohistochemistry (IHC), and Western blotting). While Biotinylated antibodies by themselves do not produce a signal that can be visualized, biotin has a high affinity and specificity to Streptavidin/Avidin. Using ImmunoReagents’ streptavidin conjugates with biotinylated antibodies results in signal amplification.

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Catalog Number: (10050-140)

Supplier: Enzo Life Sciences

Description: Researchers cloned DJ-1 protein, consisting of 189 amino acids, from a HeLa cell cDNA library as a oncogene which weakly transformed NIH3T3 cells. Cotransfection with ras or c-myc enhances transformation by DJ-11. DJ-1 expression is induced by growth signals, and it is translocated from the cytoplasm to the nucleus during the S phase without the presence of any nuclear localization signals. In addition to its potential involvement in ras-mediated signal transduction pathways, DJ-1 is known to regulate transcription of androgen-responsive genes, and is sensitive to oxidative stress. DJ-1 is ubiquitously expressed in various human tissues including the brain, with high expression in astrocytes of the frontal cortex and substantia nigra. Homozygous mutations in the DJ-1 gene have been shown to cause early-onset autosomal recessive Parkinson’s disease.

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Supplier: Enzo Life Sciences

Description: Host: Mouse, Isotype: IgG1

Catalog Number: (RL708-401-002)

Supplier: Rockland Immunochemical

Description: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

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Catalog Number: (10150-062)

Supplier: Immunoreagents

Description: ImmunoReagents’ Biotinylated antibody conjugates are widely used due to their ability to enhance antibody signal in most immunoassays. Biotinylated secondary antibodies are used in most immunoassay formats (i.e., ELISA, Immunohistochemistry (IHC), and Western blotting). While Biotinylated antibodies by themselves do not produce a signal that can be visualized, biotin has a high affinity and specificity to Streptavidin/Avidin. Using ImmunoReagents’ streptavidin conjugates with biotinylated antibodies results in signal amplification.

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Catalog Number: (RL606-1104)

Supplier: Rockland Immunochemical

Description: Secondary Goat Anti-IgG F(ab')2 Reacts with Guinea Pig

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Catalog Number: (10150-102)

Supplier: Immunoreagents

Description: TRITC (Tetramethylrhodamine isothiocyanate) is a popular rhodamine derivative pink in color but emits a red-orange color upon Emission at 580nm. TRITC is frequently paired with FITC in double labeling experiments and conjugated antibodies are often used in immunofluorescence microscopy, flow cytometry, FLISA (fluorescent ELISA) and high throughput screening assays. These conjugates are best excited with the 532 nm spectral line of the He-Ne laser and are also recommended as a second color detection reagent in in situ hybridization applications. (Excitation/Emission = 555nm / 580nm Emission Color = Orange to Red (Similar Dyes: Alexa Fluor 555, Cy3, DyLight 550)

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Catalog Number: (95019-218)

Supplier: GE Healthcare - Life Sciences

Description: For use in ELISA systems
For ELISA, it is recommended that the conjugate should be diluted in the range 1:4000 to 1:8000
Amdex conjugates are already supplied as components of the Biotrak high-sensitivity cytokine ELISAs, complete with fully-optimized protocols
Easily soluble in cold and hot water

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Catalog Number: (RL600401A76S)

Supplier: Rockland Immunochemical

Description: Protein kinase, cell signaling, cancer Primary Antibody tested for use in ELISA and western blotting. Expect a band approximately 76 kDa in size corresponding to PKC beta by western blotting in the appropriate cell lysate or extract.25ul

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Catalog Number: (RL010-0143)

Supplier: Rockland Immunochemical

Description: Produced through a multi-stage process that includes delipidation, salt fractionation, ion-exchange chromatography, gel filtration, and affinity chromatography. No contaminating proteins are observed when assayed at a protein concentration of 20mg/mL against anti-whole serum or anti-fragment specific antisera. All immunoglobulin fragments are prepared from highly purified, whole molecules subject to enzymatic digestion.

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Supplier: Enzo Life Sciences

Description: The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Catalog Number: (RL600-401-C04)

Supplier: Rockland Immunochemical

Description: Anti-ELOVL6 Antibody is suitable in ELISA, WB, and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~29 - 31 kDa in size corresponding to ELOVL6 by Western blotting in the appropriate cell lysate or extract.

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