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Supplier: MP Biomedicals

Description: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: MP Biomedicals

Description: Chlortetracycline hydrochloride has antimicrobial action similar to tetracycline hydrochloride, but it is somewhat less active against many Gram-negative organisms. It was first isolated from the culture of <i>Streptomyces aureofacien</i>.

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Catalog Number: (10018-196)

Supplier: Biotium

Description: DEAC-dUTP (diethylaminocoumarin-dUTP) can be used to synthesize fluorescently labeled DNA probes for in-situ hybridization, microarray or blotting techniques.

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Supplier: Healthcare Products

Description: Tegaderm™ Silicone foam border dressing is soft, conformable, and comfortable to wear.

Supplier: Strem Chemicals Inc

Description: CAS #: 7761-88-8. Size: 10g.

Supplier: MP Biomedicals

Description: Cycloheximide is a glutarimide antibiotic derived from a microbial source. Cycloheximide is an antibiotic which is very active against many molds, yeasts, and phytopathogenic fungi. It exhibits somewhat lower activity against bacteria and certain fungi. Control of various molds and fungi in gelatin-based photographic emulsions, photoengraving glues, and other light-sensitive products is suggested.
Cycloheximide is used in plant research to study disease resistance and as an ethylene stimulant, useful in studies involving fruit and leaf production. It is also used in bacteriological media to isolate or count bacteria in the presence of yeast and molds; Used in protein synthesis in apoptosis; Gene expression; Glycogenolysis, gluconeogenesis and ureogenesis in isolated rat hepatocytes; Studies involving steroidogenesis; Used in plant regulation and as a quality control measure by the food and beverage industry.
Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors. Cycloheximide inhibits peptide synthesis in eukaryotic organisms but not in prokaryotes. Protein synthesis is blocked by the interaction of cycloheximide with the translocase enzyme. This interaction prohibits the translocation of messenger RNA on the cytosolic, 80S ribosomes without inhibiting organelle protein synthesis. Cycloheximide is also known to induce FAS/FAS Ligand apoptosis, and triggers apoptosis in HL-60 cells, T-cell hybridomas, Burkitt's lymphoma cells in addition to a variety of other cell types. Cycloheximide will also delay or inhibit apoptosis induced by other agents.

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Supplier: Spectrum Chemicals

Description: Calcium Phosphate Tribasic, Powder, Reagent can also be referred to as Tricalcium phosphate and is abbreviated TCP. It is one of the main products of bone combustion but can also be obtained from inorganic sources such as mineral rock. It is used in baby powder as well as in powdered spices as an anti-caking agent. It is also used in gene transfection as well as for repairing bony defects and as a diet supplement. It has also historically been used in the production of porcelain. Its reagent grade means this is the highest quality commercially available for this chemical and that the American Chemical Society has not officially set any specifications for this material.

Supplier: HiMedia

Description: It is a certified grade manufactured under controlled condition by autolysis of <i>Saccharomyces cerevesiae</i>

SDS

Supplier: MP Biomedicals

Description: Lecithin is the major membrane phospholipid in eukaryotic cells. It also serves as a reservoir for several lipid messengers.

SDS

Supplier: Tronex

Description: Advanced natural rubber latex formulation provides superior elasticity and comfort for reduced hand fatigue, as well as excellent barrier protection.

Catalog Number: (100264-646)

Supplier: Indofine Chemical Company

Description: Herbal Standards 134-04-3 10mg 3,5,7,4'-Tetrahydroxyflavylium chloride 306.71 Hydroscopic.

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Supplier: MP Biomedicals

Description: Vancomycin inhibits bacterial mucopeptide biosynthesis

SDS

Supplier: MP Biomedicals

Description: Soluble in alcohol, ether, methanol (10 mg/mL - clear, yellow solution), ethanol(50 mg/mL), acetone; very slightly soluble in chloroform; almost insoluble in water.

SDS

Supplier: MP Biomedicals

Description: Soluble in water (357 mg/mL at 25 °C; 384 mg/mL at 100 °C), glycerol (100 mg/mL); very slightly soluble in ethanol. Solubility in water is decreased by HCl; almost insoluble in concentrated HCl. Aqueous solutions are at a neutral pH (6.7 - 7.3). Aqueous solutions of sodium chloride are stable at room temperature.

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Supplier: MP Biomedicals

Description: p-Nitrophenol is used as an indicator in 0.1% alcohol solutions. 4-Nitrophenol is used as a precursor to prepare phenetidine, acetophenetidine and pH indicator. Its carboxylate ester derivatives are involved as an active component for the construction of amide moieties in peptide synthesis.

SDS Certificates

Supplier: MP Biomedicals

Description: Methotrexate is a cell cycle arresting agent with varying effects. Methotrexate has been reported to arrest the cell cycle in late G1/S thus leading to the inhibition of the synthesis of DNA, RNA, thymidylates, and proteins. The main mechanism of action is reported to involve the inhibition of enzymes involved in purine metabolism which leads to the accumulation of adenosine or the suppression of intercellular adhesion molecule expression by T cells. Additionally, this compound has been observed to inhibit DHFR.
Methotrexate is used for chemotherapy either alone or in combination with other agents. It is effective for the treatment of a number of cancers including: breast, head and neck, leukemia, lymphoma, lung, osteosarcoma, bladder, and trophoblastic neoplasms. It is also used in treatment of autoimmune diseases, ectopic pregnancy, and for the induction of medical abortions. It is used to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites.
Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.
Methotrexate is an allosteric inhibititor of dihydrofolate reductase (DHFR), the enzyme that catalyzes the conversion of dihydrofolate to tetrahydrofolate. Since tetrahydrolfolate is required for purine and pyrimidine synthesis, methotrexate treatment results in the inhibition of DNA and RNA synthesis.