Catalog Number:
(77447-776)
Clearance
Supplier:
Sino Biological
Description:
A DNA sequence encoding the SARS-CoV-2 (BA.2.75) Spike S1+S2 trimer (YP_009724390.1, with mutations T19I, L24del, P25del, P26del, A27S, G142D, K147E, W152R, F157L, I210V, V213G, G257S, G339H, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, G446S, N460K, S477N, T478K, E484A, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, F817P, A892P, A899P, A942P, Q954H, N969K, K986P, V987P and furin cleavage site mutants) was expressed with bacteriophage T4 fibritin and a C-terminal polyhistidine tag followed by an AVI tag. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.2.75).The expressed protein was biotinylated <i>in vivo</i> by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.