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T3 RNA Polymerase, Promega

Catalog Number: (PAP2083)
Supplier: Promega Corporation
Description: DNA-dependent RNA polymerase for in vitro transcription.

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Product Image-102855-166

E.coli RNA Polymerase, Core Enzyme, New England Biolabs

Catalog Number: (102855-166)
Supplier: New England Biolabs (NEB)
Description: E.coli RNA Polymerase Core Enzyme consists of 5 subunits designated a, a, b´, β, and ω. The enzyme is free of sigma factor and does not initiate specific transcription from bacterial and phage DNA promoters. The enzyme retains the ability to transcribe RNA from nonspecific initiation sequences. Addition of sigma factors will allow the enzyme to initiate RNA synthesis from specific bacterial and phage promoters. The core enzyme has a molecular weight of approximately 400 kDa.

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Product Image-102855-168

E.coli RNA Polymerase, Holoenzyme, New England Biolabs

Catalog Number: (102855-168)
Supplier: New England Biolabs (NEB)
Description: E.coli RNA Polymerase, Holoenzyme is the core enzyme saturated with sigma factor 70. The Holoenzyme initiates RNA synthesis from sigma 70 specific bacterial and phage promoters.

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Product Image-97064-716

Phenol 99,0 saturated with buffer pH 4.5

Catalog Number: (97064-716)
Supplier: VWR
Description: For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation. VWR’s phenol products are manufactured from the purest crystalline phenol to ensure optimal results.

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Product Image-PAP1081

SP6 RNA Polymerase, Promega

Supplier: Promega Corporation
Description: DNA-dependent RNA polymerase for in vitro transcription.

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Image Unavailable-PAP2077

T7 RNA Polymerase, Promega

Supplier: Promega Corporation
Description: DNA-dependent RNA polymerase for in vitro transcription.

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Image Unavailable-PAP4074

T7 RNA Polymerase (HC), Promega

Catalog Number: (PAP4074)
Supplier: Promega Corporation
Description: DNA-dependent RNA polymerase for in vitro transcription.

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Product Image-97064-064

VWR® Bovine Ribonuclease A (from Pancreas)

Supplier: VWR
Description: Ribonuclease A is an endoribonuclease that efficiently hydrolyzes RNA contaminants in DNA preparations from tissue or bacterial cell cultures. RNase A is used for a variety of molecular biology applications, most commonly for the hydrolysis of RNA that contaminates DNA preparations. This enzyme is used during plasmid purification without nicking or degrading plasmid.

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Product Image-97061-078

Uridine-5'-triphosphoric acid trisodium salt (UTP-Na3), Ultra Pure Grade

Catalog Number: (97061-078)
Supplier: VWR
Description: VWR offers riboneucleotides for amplification of RNA in in vitro transcription.

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Product Image-76407-350

Hi-T7 RNA Polymerase (High Concentration), New England Biolabs

Catalog Number: (76407-350)
Supplier: New England Biolabs (NEB)
Description: Hi-T7 RNA Polymerase (High Concentration) is offered at a 20-fold higher concentration than our standard Hi-T7 RNA Polymerase and is ideal for experienced users interested in building and optimizing their own <i>in vitro</i> transcription reactions.
Product Image-76081-618

R&DNA™ T7 Polymerase, 'RNA Synthesis' with NTPs and dNTPs, Biosearch Technologies

Catalog Number: (76081-618)
Supplier: Lucigen
Description: Synthesize "RNA" transcripts that also contain both standard or modified dNTPs using this mutant T7 RNA polymerase
Product Image-97062-922

Ribonuclease Inhibitor (RNase Inhibitor)

Catalog Number: (97062-922)
Supplier: VWR
Description: RNase Inhibitor is used to inhibit the activity of RNases in reaction mixtures for cDNA synthesis and in vitro transcription, as well as for long term storage of valuable RNA samples

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Product Image-97062-538

VWR® Hybridization Cocktail

Catalog Number: (97062-538)
Supplier: VWR
Description: Offers blocking and hybridization for optimized complimentary base pairing of DNA and RNA in Southern blot, Northern blot, dot blot, colony and in situ hybridization.

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Product Image-470302-312

Ribonucleic Acid

Catalog Number: (470302-312)
Supplier: Ward's Science
Description: CAS Number: 63231-63-0
Solubility: Water and Alcohol
Synonyms: RNA
Shelf Life (months): 36
Storage: Green

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Product Image-97061-524

Guanidinium thiocyanate ≥99.0% for biotechnology

Supplier: VWR
Description: A powerful protein denaturant used to inactivate endogenous RNases during the isolation of RNA from tissues and bacterial cultures

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Product Image-10024-280

PROSAT® Sterile Presaturated Nonwoven Wipers, Contec®

Supplier: Contec
Description: Meltblown polypropylene wipes provide a consistent release of solvent to thoroughly remove surface contaminants in critical environments. PROSAT® presaturated wipes are exceptionally clean and also free from additives of any kind. This particular meltblown polypropylene contains very low levels of sodium and other ions.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
In order to process your orders without delay, we request that you provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide identification that includes your business name and shipping address via email CMD_NA@vwr.com or fax 484.881.5997 referencing your VWR account number . Acceptable forms of identification are:

  • State issued document with your organization's Federal Tax ID Number
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  • City or County issued Business License
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  • Any other ID issued by the State that includes the business name and address

* Please note if your account is within the State of California two of these pieces of identification will be required.
VWR will not lift restrictions for residential shipping addresses.

-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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