You Searched For: Cloning Buffers

Description: For the quantitative determination of active NFκB p65 in cell lysates of human, mouse, and rat origin.

The NFκB p65 EIA kit provides a sensitive, rapid and reliable method to measure the active form of NFκB p65. The assay uses streptavidin-coated plates with bound NFκB biotinylated-consensus sequence to capture only the active form of NFκB. The captured active NFκB is incubated with a specific NFκB p65 antibody, which is then detected using an HRP conjugated secondary antibody. The assay is developed with a chemiluminescent substrate and the signal is detected using a luminometer. Higher throughput 96 well plate based method faster than EMSA and Western blot with sensitive chemiluminescent detection.

Catalog Number: 76218-676

Supplier: Enzo Life Sciences

Sale

Description: Instatherm® is a safer, more precise heating method for the laboratory

Catalog Number: 71000-040

Supplier: Ace Glass

Sale

Description: BAKER ULTRA RESI-ANALYZED® Reagent—ultra high-purity solvents and salts developed for organic residue extraction/concentration procedures.

Catalog Number: JT9254-2

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

Certificates Sale

Description: Fluor de Lys® Substrate is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in a second step, treatment with the Fluor de Lys® Developer produces a fluorophore. Histone deacetylases from all known classes have been successfully assayed with the Fluor de Lys® Substrate. These include human HDACs 1, 2, 3 and 8 (class I), human HDACs 4 and 9 (class II) and the sirtuins yeast Sir2, human SIRT1 and human SIRT2 (class III; reaction must include the sirtuin substrate NAD+). The substrate is cell-permeable and serves as the basis for the HDAC Cellular Activity Assay Kit (Prod. No. BML-AK503). NOTE: Must be used in conjunction with Fluor de Lys® Developer (Prod. No. BML-KI105). Sufficient for 100-1000 assays of HDACs or sirtuins (50 µl reaction volume, 50-500 µM (substrate).

Catalog Number: 89160-282

Supplier: Enzo Life Sciences

Sale

Description: KITLG is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis.This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene.

Catalog Number: 10102-860

Supplier: Prosci

Sale

Description: BIOMOL Green™ provides a simple and convenient method for colorimetric phosphate quantitation (abs. 600-680 nm). Unlike other molybdate/malachite green-based assays, BIOMOL Green™ doesn’t require multiple solutions or reagents prepared fresh on the day of the assay. BIOMOL Green™ is extremely stable (>6 mos. at 4°C) and is simply mixed, at room temperature, with any enzymatic reaction that has released free phosphate. Reported applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase.BIOMOL Green™ has perhaps been most widely applied to protein phosphatase assays. Typically, a protein phosphatase (e.g. PTP1B or LAR) is simply incubated with an appropriate phosphopeptide (e.g. IR5,9,10). Addition of BIOMOL Green™ stops the reaction and begins color development, which is read 20-30 min. later.BIOMOL Green™ may be used in cuvette or microplate-based assays and is ideal for high-throughput applications. Each kit comes with a standardized phosphate solution for assay calibration.

Catalog Number: 89156-426

Supplier: Enzo Life Sciences

Sale

Description: OPTI-GUARD® sets the standard for extremely low impact, easy to use pre-column protection. Designed for use with analytical (Int.Ø: 4.6, 3.0 mm) and narrow-bore (Int.Ø: 2.1, 1.0 mm) columns, the patented floating stem design automatically adjusts to any manufacturer's tube stop depth for a zero-dead-volume connection every time.

Catalog Number: 77100-028

Supplier: Optimize

Sale

Description: RNA-Solv® Reagent is a one reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is a modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenised and lysed in RNA-Solv® Reagent, which maintains the integrity of the RNA while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

Catalog Number: 76509-254

Supplier: Omega Bio-Tek

Sale

Description: Accurately monitor pH, ion concentration, mV, ORP and temperature on two channels with the ORION™ Dual Star™ Benchtop Meter for advanced laboratory analysis.

Catalog Number: 97039-500

Supplier: Thermo Scientific

Sale

Description: AR Select—A line of acids specifically developed for trace metal analysis; analyzed for up to 32 metals in the low ppb range. 

Catalog Number: MK662346

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

SDS Certificates Sale

Description: AR Select®—A line of acids specifically developed for trace metal analysis; analyzed for up to 32 metals in the low ppb range. 

Catalog Number: MK666546

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

SDS Certificates Sale

Description: Equivalence C18 columns have been specifically developed as a high quality, cost effective replacement for many HPLC column brands.

Catalog Number: 10829-210

Supplier: HICHROM LIMITED

Sale

Description: Solid Phase Extraction (SPE) is commonly used as a highly selective sample clean-up or sample enrichment method prior to subsequent analyses. J.T.Baker® BAKERBOND® SPE 96 well plates are well suited for high throughput and automated SPE methods. With complementary high capacity sorbents to existing spe extraction columns in the J.T.Baker® BAKERBOND® range, higher throughput methodologies can now be simply developed and automated.

Catalog Number: JT7020-P10

Supplier: HICHROM LIMITED

Sale

Description: BAKERBOND® Q2100 LC phases have been specially designed to solve the challenges of most routine samples. They can also be a good starting point for new method development projects. With options of four widely used chemistries and three particle sizes BAKERBOND® Q2100 can be a useful addition to your analytical toolkit.

Catalog Number: JT7358-42

Supplier: HICHROM LIMITED

Sale

Description: J.T.Baker® volumetric and analytical solutions. These solutions are suitable for use in ACS, USP and NF compendial methods and general laboratory applications. 

Catalog Number: JT5671-7

Supplier: AVANTOR PERFORMANCE MATERIAL LLC

Certificates Sale

Description: The Avantor® ACE® Excel® C18-AR phase utilizes a specially developed ligand combining a C18 chain with integral phenyl functionality, thus combining the benefits of both C18 and phenyl characteristics into a single phase. This can be used for ‘standard’ C18 column separations, but is additionally recommended for separations that involve compounds containing aromatic functionality.

Catalog Number: 76382-642

Supplier: HICHROM LIMITED

Sale