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The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Catalog Number: (102715-942)
Supplier: New England Biolabs (NEB)
Description: ElectroLigase™ combines T4 DNA ligase and an optimized, ready-to-use 2X reaction buffer containing a proprietary ligation enhancer and no PEG.

Small Business Enterprise


Supplier: Promega Corporation
Description: T4 DNA Ligase and buffer combination for rapid (5-minute) ligations.
Supplier: New England Biolabs (NEB)
Description: NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly.
Catalog Number: (200064-420)
Supplier: New England Biolabs (NEB)
Description: 9°N™ DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotides which are hybridized to a complementary target DNA

Small Business Enterprise


Catalog Number: (PAC8820)
Supplier: Promega Corporation
Description: The Flexi Vector System provides a rapid, efficient, high-fidelity way to transfer protein-coding regions without the need to resequence.

Catalog Number: (PAP2301)
Supplier: Promega Corporation
Description: NM522 contains an F' episome, which is required for production of ssDNA and for blue/white color selection.


Catalog Number: (102877-808)
Supplier: New England Biolabs (NEB)
Description: The NEBuilder HiFi DNA Assembly Cloning Kit combines the power of the NEBuilder HiFi DNA Assembly Master Mix with NEB 5-alpha Competent E

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Supplier: New England Biolabs (NEB)
Description: Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.

SDS Small Business Enterprise

Supplier: New England Biolabs (NEB)
Description: Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA ligase and a proprietary ligation enhancer in an optimized reaction buffer.

Small Business Enterprise

Catalog Number: (PAC8640)
Supplier: Promega Corporation
Description: The Flexi Vector System provides a rapid, efficient, high-fidelity way to transfer protein-coding regions without the need to resequence.


Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, truncated (T4 Rnl2 truncated) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA.

Small Business Enterprise

Catalog Number: (PAP9751)
Supplier: Promega Corporation
Description: Useful host for transformation of pGEM Vectors and production of ssDNA from M18 or phagemid vectors. JM109 is recA- and lacks the E. coli K restriction system. Can be used for blue/white screening.

Catalog Number: (PAG6050)
Supplier: Promega Corporation
Description: HaloTag Cloning Starter Kit: designed to use Flexi Cloning System to clone protein coding sequences into mammalian HaloTag expression vectors; clone and transfer gene sequences into C- or N-terminus of HaloTag Flexi CMV Deletion Vectors.


Supplier: New England Biolabs (NEB)
Description: The NEBNext Ultra II Ligation Module has been optimized to efficiently ligate DNA adaptors compatible with Illumina® sequencing to end-repaired, dA-tailed DNA fragments

Small Business Enterprise

Catalog Number: (103307-256)
Supplier: New England Biolabs (NEB)
Description: RtcB Ligase is expressed as a His-tagged fusion in <i>E. coli</i>.

Small Business Enterprise


Supplier: New England Biolabs (NEB)
Description: A salt tolerant variant of T4 DNA Ligase, Salt-T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA and is designed to function at higher salt concentrations than wild type T4 DNA Ligase.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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