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You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Product Image-99900-316

Gigapack III Packaging Extracts, Agilent Technologies

Supplier: Agilent Technologies
Description: For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highly methylated DNA. Each packaging extract is restriction minus to optimize packaging efficiency and library representation.

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Mutagenesis Kits QuikChange, Agilent Technologies

Supplier: Agilent Technologies
Description: The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 -14 kb) or otherwise difficult-to mutagenize plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.

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BioXp® Select DNA Cloning Golden Gate Assembly BsmBI Kits

Supplier: SGI - DNA Synthetic Genomics
Description: The Select DNA cloning Golden Gate Assembly BsmBI kits are compatible with the Type IIS restriction enzymes BsmBI. Please choose the appropriate kit based on the number of fragments being assembled.

New Product

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BioXp® Select DNA Cloning and Amplification Kits

Supplier: SGI - DNA Synthetic Genomics
Description: The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp® 3250.

New Product

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Gibson Assembly® ULTRA Kits and Master Mixes (2X)

Supplier: SGI - DNA Synthetic Genomics
Description: Generate seamless plasmid DNA or BAC clones without restriction digestion.

SDS

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NEB Cloning Competent E. coli Sampler, New England Biolabs

Catalog Number: (76299-644)
Supplier: New England Biolabs (NEB)
Description: The NEB Cloning Competent E.coli Sampler allows to sample four of popular chemically competent strains.
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BioXp® Select DNA Cloning Golden Gate Assembly, BsaI Kits

Supplier: SGI - DNA Synthetic Genomics
Description: The Select DNA cloning golden gate assembly, BsaI kits are compatible with the Type IIS restriction enzymes BsaI. Please choose the appropriate kit based on the number of fragments being assembled.

New Product

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BIOXP® 9600 Select Clone and AMP

Supplier: SGI - DNA Synthetic Genomics
Description: The Select DNA cloning and amplification kits generate a minimum of 10 µg of amplified DNA from fragments of 0.3 to 1.8 kb using the BioXp 9600.

New Product

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BIOXP® Select Plasmid AMP

Supplier: SGI - DNA Synthetic Genomics
Description: BioXp Select plasmid amplification kits contain reagents for isothermal amplification of plasmid DNA.

New Product

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Gibson Assembly® HIFI 1-Step Kits and Master Mixes (2X)

Supplier: SGI - DNA Synthetic Genomics
Description: Generate seamless plasmid DNA or BAC clones without restriction digestion.

SDS

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NEB PCR Cloning Kit (Without Competent Cells), New England Biolabs

Catalog Number: (102969-096)
Supplier: New England Biolabs (NEB)
Description: This PCR Cloning Kit contains an optimized Cloning Mix containing a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background.
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NEB® Golden Gate Assembly Kits (BsaI-HF®v2), New England Biolabs

Supplier: New England Biolabs (NEB)
Description: This NEB Golden Gate Assembly Kit (BsaI-HFv2) contains an optimized mix of BsaI-HFv2 and T4 DNA Ligase. Together these enzymes can direct the assembly of multiple inserts/modules using the Golden Gate approach. The pGGA destination plasmid (included), provides a backbone for your assembly, features convenient restriction enzyme sites for subcloning, and has T7/SP6 promoter sequences to enable <i>in vitro</i> transcription.

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Pfu DNA Ligase, Agilent Technologies

Catalog Number: (99902-306)
Supplier: Agilent Technologies
Description: <i>Pfu</i> DNA ligase is a recombinant enzyme derived from <i>Pyrococcus furiosus</i> hyperthermophilic marine archaebacterium that catalyzes linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.
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QUICK BLUNTING™ and QUICK LIGATION™ Kits, New England Biolabs

Catalog Number: (101417-934)
Supplier: New England Biolabs (NEB)
Description: NEB offers the Quick Blunting™ and Quick Ligation™ Kits together as a bundle at a value price.
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StrataClone PCR Cloning Kits, Agilent Technologies

Supplier: Agilent Technologies
Description: The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase.

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DNA Ligase T4

Supplier: Rockland Immunochemical
Description: DNA Ligase T4 Molecular Biology Grade

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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