Proteinase K
Supplier: Jopak VerpakkingenTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
IBI's Proteinase K is a stable >50 mg/ml (powder) highly active proteolytic enzyme that is purified from the mold tritirachium album.
IBI's Proteinase K Solution is a stable 20 mg/ml solution. The high activity (0.6 U/µl) means you can keep the reaction volume as low as 200 mg/ml. The solution is composed of 10 mM Tris, 1 mM Calcium Chloride, and 30% Glycerol with the pH adjusted to 8.0 with HCl.
Store solution at +4 °C and keep tightly sealed. Store powder at –20 °C and warm to room temperature before opening. Dnase and Rnase Free.
The enzyme has two binding sites for Ca++, which lie some distance from the active site, and is not directly involved in the catalytic mechanism. The high activity of the enzyme solution (0.6 U/µl) means that you can expect more complete digestion of your sample.
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