Dithiothreitol (DTT, Cleland's reagent) for biotechnology

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Dithiothreitol (DTT, Cleland's reagent) for biotechnology

Dithiothreitol (DTT, Cleland's reagent) for biotechnology

0281-25G 0281-5G

97061-338CS 5296.86 USD

97061-338 97061-340

Dithiothreitol (DTT, Cleland's reagent) for biotechnology

Dithiothreitol (DTT, Cleland's reagent)

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Formula: C₄H₁₀O₂S₂
Boiling Pt: ∼125 °C (1013 hPa)
Melting Pt: 42 C…43 °C
Flash Pt: >110 °C

MDL Number: MFCD00004877
CAS Number: 3483-12-3

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