Mag-Bind® Endo-free Plasmid Midi Kits
Supplier: Bierbaum-Proenen GmbH & Co.KGTotal Ratings: 0
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The Mag-Bind® Endo-free Plasmid Midi Kit is designed to deliver large scale purification of high-quality plasmid DNA with endotoxins levels <0.1 EU/µg for use in eukaryotic transfections and other sensitive in vitro applications.
- Fully automatable - Magnetic beads clear the lysate, avoiding the manual centrifugation step
- High-yielding - Plasmid DNA yield up to 200 μg
- Rapid - 24 samples can be processed in ~70 minutes starting from a bacterial pellet without manual intervention
- Versatile - Process up to 50 mL bacterial culture with different plasmid types and culture medias
- Transfection-quality - Endotoxin free plasmid DNA with endotoxins levels <0.1 EU/µg
- Pure - Contamination-free plasmid DNA with A260/A280 >1.8 and A260/A230 >1.8
The Kit follows magnetic bead-based technology to purify plasmid DNA from up to 50 ml bacterial culture in LB or up to 10 ml bacterial culture in TB or other suitable growth medium. The Mag-Bind® Endo-free Plasmid DNA Kit can be processed either manually or automated on most open-ended liquid handling platforms as well as magnetic processors. Yields may vary depending on the copy number of the plasmid, E. coli strain used, culture media, and condition of growth.
The Mag-Bind® Endo-free Plasmid Midi Kit follows a modified alkaline lysis method forming a bacterial lysate. The lysate is then cleared using either Mag-Bind® Particles LC (sold separately) or Lysate Clearance Filter Syringe (sold separately) or centrifugation. The novel paramagnetic lysate clearance beads bind to cell debris and pull them down when magnetized. This eliminates the need for centrifugation or vacuum manifold for lysate clearance, making this suitable for high-throughput, automation workflows.
Post lysate clearance, plasmid DNA is then bound to Mag-Bind® Particles RQ through a specially formulated binding buffer and the Mag-Bind® particles are subjected to a wash routine to remove endotoxins, salt, and other contaminants. High-quality plasmid DNA is then eluted in Endo-free Water and is ready for use in a wide range of downstream applications such as transfection routine screening, restriction enzyme digestion, transformation, PCR, and DNA sequencing and more.
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