NANOLITER2020 is the updated version of WPI’s popular NANOLITER2010 Injector that is capable of performing precise injections down to 0.6 nl repeatibly, and can be paired with a variety of glass micropipette sizes.
- Perform nanoliter volume injections down to 25 nl using glass micropipettes
- Fine control of plunger displacement along with proper sealing among gasket, glass micropipette, and oil ensures precision and accuracy
- Control up to two pumps simultaneously
- Easily mounted on a micromanipulator or a stereotaxic frame
- Precise control over injection volumes (in the nanoliter to microliter range) and injection rates
- Simple to use touchscreen interface and graphical representation of volume status on MICRO2T controller
- LED indicator offers a visual indication of connectivity
- Perform injections with a foot switch (optional)
- Injector is compatible with different OD glass micropipettes (in the range of 1.1 to 1.5 mm)
The new model connects directly to the WPI MICRO2T SMARTouch™ controller, which can also be used with WPI’s UMP3 pump, allowing the pumps to be controlled individually or synchronously for a variety of low-volume injection applications.
Application: The NANOLITER2020 Injector is perfect for making nanoliter to microliter range injections into frogs (Xenopus oocytes), rats, mice, mosquitoes, shrimps, insects (e.g., Brown Planthopper), flies (e.g., Drosophila) and fish (Zebrafish) embryo using glass micropipettes.
Injections performed with mineral oil back-filled glass micropipettes, and the sample is front filled. This approach minimizes loss of any costly and scarce sample when a minute sample volume can be adequate.
Nanoliter Injector works on the principle of Positive Displacement. An internal micrometer step motor precisely moves the metal plunger which pushes the oil inside the micropipette, and the oil-layer pushes nanoliter volumes of aqueous sample out of the pipette tip.
NANOLITER 2020 plunger outer diameter: 482 µm
Plunger movement for 100 nl volume dispense: 550 µm ±55 µm
Piston movement per dispensed volume (nl): 5.5 µm/nl
Linear travel per full step: 12.7 µm/step
Injection volume: 4200 nl (Maximum), 12.5 nl (Minimum)
Maximum rate: 644 nl/sec
Glass use capabilities: 1.10 to 1.15 mm OD fire-polished glass with green front gasket, 1.30 to 1.35 mm OD fire-polished glass with black front gasket, 1.5 mm OD fire-polished glass with red front gasket
Recommended glass: Micropipette, 1.14 mm OD fire-polished glass capillaries
Recommended minimum injection volume: 25 nl with 1.14 OD glass (Green front gasket), 50 nl with 1.5 mm OD glass (1.5 mm OD fire-polished glass and red front gasket)
Ordering information: NANOLITER2020 is the complete system. 300704 is the NANOLITER2020 injector head only, and the controller and the accessories are not included.
