HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid free acid ≥99.0%

Supplier: Jopak Verpakkingen
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IB01131EA 117 USD
IB01131 IB01132 IB01133 87004-972
HEPES-2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid free acid ≥99.0%
HEPES (4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid)

N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic Acid


  • For use in polyacrylamide electrophoresis buffer systems
  • Idealcell culture buffer
  • Maintains physiological pH in cell culture applications


Ideally suited for cell culture in a range of 10–25 mM. The zwitterionic buffer has a buffering ranges of 6.8–8.2. It can also be used as a component of TRIS-HEPES buffer for polyacrylamide electrophoresis of proteins. DNase-, RNase-, and protease-free.


Caution: Store at room temperature. Protect from moisture. Irritant. Avoid contact with eyes, skin, and mucous membranes. Avoid breathing vapors. Wash thoroughly with water after handling.

Formula: C₈H₁₈N₂O₄S
MDL Number: MFCD00006158
CAS Number: 7365-45-9
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Specification Test Results

Appearance White Crystalline Powder
pH (0.5 M solution in H₂O): 5.1 to 6.5
Purity Min. 99.0
Water Max. 0.25%
Heavy Metals Max. 0.001%
Iron Max. 0.0005%
Sulfates Max. 0.05%
DNase assay None Detected
RNase assay None Detected
Protease assay None Detected
Cell Culture Testing Pass

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