Radiant™ Hot-Start 2X Taq Master Mix
Supplier: ALKALI SCIENTIFIC MSTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
Enhance PCR efficiency with Radiant™ Hot-Start 2X Taq master mix, providing optimal results from the start.
- New-generation PCR formulation including enhancers for maximum PCR efficiency, sensitivity and reaction speed
- Robust PCR performance across a wide range of DNA templates including multiplex assays and GC-rich and AT-rich sequences
- Supplied as a ready-to-use 2X Mastermix for maximum convenience and minimal liquid handling
- High-yields with amplicons up to 5 Kb with standard or fast cycling
Radiant™ 2X Hot-Start Taq Mastermix is a ready-to-use, robust PCR mix containing highly purified Hot-Start Taq DNA Polymerase, reaction buffer, ultra-pure dNTPs, MgCl₂ and PCR enhancers. Radiant™ 2X Hot-Start Taq Mastermix is optimized for the sensitive DNA amplification of a wide range of DNA templates including complex mammalian genomic DNA and crude samples. The Hot-Start technology is based on a new-generation PCR buffer formulation in conjunction with a antibody-mediated chemistry. Radiant™ Hot-Start Taq Mastermix exhibits 5´ to 3´ DNA polymerase activity with an error-rate of wild-type Taq (2.0×10-5). The polymerase mix is ideal for genotyping, colony PCR, multiplexing, low-copy assays, and the amplification of challenging targets susceptible to mispriming.
Radiant™ Hot start Taq Mastermix is engineered for robust, superior PCR and is supplied as a ready-to-use 2X mix which provides exceptional sensitivity and ease of use. Storage RadiantTM 2X Hot-Start Taq Mastermix is shipped on blue or dry ice and should be stored at –20 °C upon receipt. Excessive freeze/thawing should be avoided. When stored as specified, Radiant™ 2X Hot-start Taq Mastermix is stable for 12 months from date of receipt.
The kit may also be stored at 4 °C for 1 month. Important Considerations Radiant™ 2X Hot-Start Taq Mastermix: The 2X Mastermix is comprised of Radiant™ Hot-Start Taq DNA Polymerase, 2 mM dNTPs, 6 mM MgCl₂, and PCR enhancers for maximum efficiency, sensitivity and success with difficult amplicons. We do not suggest the use of additional PCR enhancers. Template: For complex genomic DNA, we suggest the use of 5 to 500 ng per reaction; For cDNA or plasmid DNA, please use <100 ng per reaction. Primers: Primers should have a predicted melting temperature of around 60 °C, using default Primer 3 settings. The final primer concentration in the reaction should be between 0.2 and 0.6 μM. Annealing: We recommend performing a temperature gradient to determine the optimal annealing temperature. Alternatively, we suggest a 55 °C annealing temperature.
Increase in 2 °C increments if non-specific products are present. Extension: Optimal extension is achieved at 72 °C. The optimal extension time is dependent on amplicon length and complexity. 15 seconds per kilobase (Kb) is recommended for amplification from eukaryotic genomic DNA or cDNA. For shorter amplicons, a 1 second extension is sufficient. For Multiplex PCR, we suggest an initial annealing temperature gradient from 55 to 65 °C in order to determine the highest level of specificity. In addition, we recommend an initial extension time of 90 seconds and greater to maximize yield and specificity. Quality Control Radiant™ 2X Hot-start Taq Mastermix is tested extensively for robust activity, processivity, efficiency, heat activation, sensitivity, absence of nuclease contamination and absence of nucleic acid contamination. Radiant™ 2X Hot-Start Taq Mastermix is manufactured under a comprehensive quality management system, following ISO 9001:2008 standards.
Caution: Limitations of use: This product is intended for research purposes only and is not intended for any animal or human therapeutic use.
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