Radiant™ 2X RED Taq Master Mix
Supplier: ALKALI SCIENTIFIC MSTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
Enhance PCR specificity with Radiant™ 2X RED Taq master mix.
- Inert red dye will not inhibit PCR reaction and is used as a downstream gel loading dye
- No change in PCR cycling conditions when in supplied red format
- New-generation PCR formulation including enhancers for maximum PCR efficiency, sensitivity and reaction speed
- Robust PCR performance across a wide range of DNA templates including genomic DNA and GC-rich and AT-rich sequences
- Supplied as a ready-to-use Red 2X Mastermix for maximum convenience and minimal liquid handling
- High-yields with amplicons up to 5 Kb with standard or fast cycling
Radiant™ Taq DNA polymerase description Radiant™ Taq DNA Polymerase is a highly purified, high performance DNA Polymerase optimized for the sensitive DNA amplification of a wide range of DNA templates including complex mammalian genomic DNA. RadiantTM Taq DNA Polymerase exhibits 5´ to 3´ DNA polymerase activity with an error-rate of wild-type Taq (2.0×10 - 5). The polymerase is ideal for screening, colony PCR, high-throughput PCR and genotyping of problematic GC-rich and AT-rich sequences. RadiantTM Taq DNA Polymerase is engineered for robust, superior PCR and is supplied with a highly optimized, new-generation buffer system which provides exceptional sensitivity.
Storage Radiant™ Taq DNA polymerase is shipped on blue or dry ice and should be stored at –20 °C upon receipt. Excessive freeze/thawing should be avoided. When stored as specified, RadiantTM Taq DNA Polymerase is stable for 12 months from date of receipt. The kit may also be stored at 4 °C for 1 month. Important considerations Radiant™ 10X Taq reaction buffer: The 10X Taq reaction buffer contains PCR enhancers and has been optimized for maximum efficiency, sensitivity and success with difficult amplicons.
We do not suggest the use of additional PCR enhancers. Template: For complex genomic DNA, we suggest the use of 5 to 500 ng per reaction; For cDNA or plasmid DNA, please use <100 ng per reaction. Primers: Primers should have a predicted melting temperature of around 60 °C, using default Primer 3 settings. The final primer concentration in the reaction should be between 0.2 and 0.6 μM. Annealing: We recommend performing a temperature gradient to determine the optimal annealing temperature. Alternatively, we suggest a 55 °C annealing temperature. Increase in 2 °C increments if non-specific products are present. Extension: Optimal extension is achieved at 72 °C.
The optimal extension time is dependent on amplicon length and complexity. 15 seconds per kilobase (Kb) is recommended for amplification from eukaryotic genomic DNA or cDNA. For shorter amplicons, a 1 second extension is sufficient. Quality control Radiant™ Taq DNA polymerase is tested extensively for robust activity, processivity, efficiency, heat activation, sensitivity, absence of nuclease contamination and absence of nucleic acid contamination. Radiant™ Taq DNA polymerase is manufactured under a comprehensive quality management system, following ISO 9001:2008 standards.
Caution: Limitations of use: This product is intended for research purposes only and is not intended for any animal or human therapeutic use.
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