NEBNext® Enzymatic 5hmC-seq Kits

Supplier: W.u.G. Dickgießer GmbH

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E3350L E3350S

77441-292EA 3110 USD

77441-292 77441-294

NEBNext® Enzymatic 5hmC-seq Kits

Nucleic Acid Reagents Next Generation Sequencing Reagents

The NEBNext® enzymatic 5hmC-seq kit specifically detects 5hmC sites.

5hmC detection at single base resolution
Enzyme-based conversion minimizes DNA damage
0.1 to 200 ng input range
Even GC coverage
E5hmC-seq and EM-seq data can be combined

The kit includes NEBNext Ultra II library prep reagents, and 5hmC is detected using a two-step enzymatic conversion workflow that minimizes damage to DNA and allows discrimination of 5hmC from both cytosine and 5mC after Illumina sequencing. E5hmC-seq data can also be subtracted from EM-seq data, allowing determination of the precise location of individual 5mC and 5hmC sites. The kit includes the E5hmC-seq adaptor and index primers are supplied separately.

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