PureCube Co-NTA Agarose
Supplier: CUBE BIOTECHTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
Our PureCube Co-NTA agarose resins are tiny agarose resin beads with a diameter of 40 µm. They are used for the purification of active His-tagged proteins from cells.
- Usage: Specific binding and purification of 6× His tagged proteins
- Specificity: Affinity to His tagged proteins
- Binding capacity: >30 mg/ml
- Bead Ligand: Co-NTA
- Bead size: 40 μm
- Filling quantity: Delivered as a 50% suspension
- Required equipment:
- Lysis Buffer
- Wash Buffer
- Elution Buffer
- Ice bath
- Refrigerated centrifuge for 50 ml tube (min 10000×g)
- 50 ml centrifuge tube
- Micropipettor and Micropipetting tips
- Disposable gravity flow columns with capped bottom outlet, 2 ml
- pH meter
- End-over-end shaker
- SDS-PAGE buffers, reagents and equipment Optional: Western -Blot reagents and equipment
Our year-long experience in manufacturing agarose resin led to a high yield of 40 mg protein per ml resin, leading in the market compared to other Co-NTA suppliers. PureCube Co-NTA resins are suited for batch spin columns and FPLC. Their small diameter provides them with excellent mechanical stability. We recommend our Co-NTA agarose beads with 100 µm diameter for higher flow rates and, for extreme cases, XL-sized Co-NTA beads. Furthermore, these beads are also available pre-packed in a column/cartridge or as magnetic beads.
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