HiScribe T7 mRNA Kit with CleanCap Reagent AG, New England Biolabs
Supplier: W.u.G. Dickgießer GmbHTotal Ratings: 0
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The HiScribe T7 mRNA Kit with CleanCap Reagent AG utilizes an optimized RNA synthesis formulation and trinucleotide cap analog technology for co-transcriptionally capping mRNAs that contain a natural Cap-1 structure in a single simplified reaction without compromising RNA yield.
- Generate up to 90 µg of Cap-1 mRNA per reaction from 1 µg of control template
- NTPs are provided separately to enable partial of full substitution of modified NTPs
- Template removal and RNA purification reagents included
- Includes linearized control template for use with CleanCap reagent AG
By using a DNA template with a T7 promoter sequence followed by an AG initiation sequence and an encoded poly(A) tail, mRNAs can be transcribed with a 5'-m7G Cap-1 structure that is polyadenylated, translationally competent and able to evade the cellular innate immune response. The kit is formatted with individual vials of NTPs and CleanCap Reagent AG to allow for partial or complete substitution of modified NTPs, with a total kit yield of 1.8 mg of mRNA. Cap-1 mRNA synthesized from this kit is suitable for many applications, including transfections, microinjections, in vitro translation, preclinical mRNA therapeutic mRNA studies as well as RNA structure and function analysis.
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