NEBNext® Globin and rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads, New England Biolabs

Supplier: W.u.G. Dickgießer GmbH
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E7755L E7755S E7755X
76377-494EA 1369 USD
76377-494 76377-496 76377-498
NEBNext® Globin and rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads, New England Biolabs
Nucleic Acid Reagents Next Generation Sequencing Reagents

The NEBNext Globin and rRNA Depletion Kit (Human/Mouse/Rat) employs the NEBNext RNase H-based RNA depletion workflow to deplete adult, embryonic and fetal globin mRNA (HBA1/2, HBB, HBD, HBM, HBG1/2, HBE1, HBQ1 and HBZ), cytoplasmic rRNA (5S, 5.8S, 18S, 28S, ITS and ETS) and mitochondrial rRNA (12S and 16S).


  • Efficient, specific depletion of rRNA and globin mRNA (adult, fetal and embryonic)
  • Suitable for low-quality or high-quality RNA
  • Compatible with a broad range of input amounts: 10 ng - 1 ug
  • Optional integration with poly(A) mRNA isolation workflows for removal of globin RNAs, rRNAs, and noIncludes Agencourt RNAClean XP beads


The kit is effective with human, mouse and rat total RNA preparations, both intact and degraded. The resulting depleted RNA is suitable for RNA-seq, random-primed cDNA synthesis, or other downstream RNA analysis. This kit can also be used following poly(A) mRNA enrichment (e.g., using the NEBNext poly(A) mRNA Magnetic Isolation Module NEB #E7490), so that the final depleted RNA contains only mRNA of interest and no non-coding RNA. For convenience, this kit includes RNA Clean beads.

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