NEBNext® Enzymatic Methyl-seq (EM-seq™) Kit, New England Biolabs

Supplier: W.u.G. Dickgießer GmbH
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E7120L E7120S
76337-038EA 3394 USD
76337-038 76337-184
NEBNext® Enzymatic Methyl-seq (EM-seq™) Kit, New England Biolabs
Microbiology Test Systems Enzymatic Tests

NEBNext® Enzymatic methyl-seq (EM-seq™) is a new method for identification of 5-mC and 5-hmC.


  • Superior sensitivity of detection of 5-mC and 5-hmC
  • Greater mapping efficiency
  • More uniform GC coverage
  • Detection of more CpGs with fewer sequence reads
  • Uniform dinucleotide distribution
  • High-efficiency library preparation, with larger library insert sizes
  • Conversion module also available separately


While bisulfite sequencing has been the gold standard for methylome analysis, this conversion treatment damages DNA, resulting in fragmentation, loss and bias. In contrast, the highly effective enzyme-based conversion in the NEBNext® Enzymatic Methyl-seq (EM-seq™) kit minimizes damage to DNA and, with the supplied NEBNext Ultra™ II library preparation workflow reagents, produces high quality libraries that enable superior detection of 5-mC and 5-hmC from fewer sequencing reads.

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