NEBNext® Enzymatic Methyl-seq (EM-seq™) Kit, New England Biolabs
Supplier: W.u.G. Dickgießer GmbHTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
NEBNext® Enzymatic methyl-seq (EM-seq™) is a new method for identification of 5-mC and 5-hmC.
- Superior sensitivity of detection of 5-mC and 5-hmC
- Greater mapping efficiency
- More uniform GC coverage
- Detection of more CpGs with fewer sequence reads
- Uniform dinucleotide distribution
- High-efficiency library preparation, with larger library insert sizes
- Conversion module also available separately
While bisulfite sequencing has been the gold standard for methylome analysis, this conversion treatment damages DNA, resulting in fragmentation, loss and bias. In contrast, the highly effective enzyme-based conversion in the NEBNext® Enzymatic Methyl-seq (EM-seq™) kit minimizes damage to DNA and, with the supplied NEBNext Ultra™ II library preparation workflow reagents, produces high quality libraries that enable superior detection of 5-mC and 5-hmC from fewer sequencing reads.
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