TotalStain Q, Fluorescent Total Protein Stain Kit, Azure Biosystems
Supplier: Fels-ChemieTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
Accurate and quantitative fluorescent total protein stain to perform normalization of Western blots.
- Fluorescent total protein stain for Western blot membranes
- Sufficient amount for 15 mini-blots
- Simple 20-minute dye-and-wash protocol
- Wide linear dynamic range between 1 to 50 μg of protein load across lysates of various tissue types
- Compatible with both chemiluminescent and near-infrared fluorescence
TotalStain Q only adds a short 20-minute step to Western blot protocol, between protein transfer and blocking buffer incubation. Western blots can be imaged on both camera-based and scanner instruments with green channel or Safe-Dyes channel. The linear range between 1 to 50 μg of lysate makes it ideal for normalization of low-expressed proteins that require high loads of protein to reach the sensitivity for the detection of these targets.
This is compatible with subsequent Western blot detection using chemiluminescence or Near-Infrared (NIR) fluorescence.
This solves the issue of housekeeping proteins that lack linearity and normalization accuracy at high protein loads.
Delivery information: Kit contains 1 bottle of staining solution, 1 bottle of washing solution and 15 units of imaging folders.
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