i7TM Hot Start High Figh Fidelity DNA Polymerase
Supplier: InstruCon, Inc.Total Ratings: 0
Avg. Ratings: 0.0 out of 5
i7™ Hot start high-fidelity DNA polymerase has the high-fidelity, sensitivity and processivity with an error rate ~2.8×102 fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes.
- Useful for long and difficult DNA amplification, high fidelity PCR, and cloning
- Supplied as convenient master mix
- Efficient for amplifying high GC template DNA with magic enhancer
- Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product.
- i7™ Hot start high-fidelity DNA polymerase
- 5X i7™ PCR buffer with Mg²⁺
- 5X Magic enhancer
i7™ hot start high-fidelity DNA polymerase is a genetically engineered, heat stable DNA polymerase which has 5' - 3' polymerase and 3' - 5' exonuclease (proof reading) activities. i7™ hot start high-fidelity DNA polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8×102-fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes in the marketplace. i7™ hot start high-fidelity DNA polymerase is ideal for cloning and can be used for long (up to 20 kb) or difficult amplicons.
Delivery information: This product is supplied with the proprietary 5X PCR reaction buffer containing MgCl₂ with a final (1X) concentration of 2 mM, and 5X magic enhancer that enables efficient amplification of GC rich templates up to 84%. All our products are shipped with dry ice.
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