Fura 2-AM 98 HPLC_ASSAY_METHOD fluorescent indicator, Calcium determination
Supplier: BIOTIUM, INCTotal Ratings: 0
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Fura-2, AM ester is a ratiometric fluorescent dye that binds to free intracellular calcium, and is the preferred dye for ratiometric imaging microscopy. Fura-2, AM ester is membrane-permeant and thus can be loaded into cells via incubation.
- Kd: 145 nM (22°C in pH 7.2 buffer) (after hydrolysis)
- λEx/λEm (no Ca2+) = 363/512 nm; λEx/λEm (high Ca2+) = 335/505 nm (after hydrolysis)
- ε (363 nm, no Ca2+) = 27,000 M-1cm-1; ε (335 nm, high Ca2+) = 35,000 M-1cm-1 (after hydrolysis)
- Cell permeability: Membrane permeant
- Light yellow solid soluble in DMSO
- Store at -20°C and protect from light, especially in solution
Because of the relatively low water solubility of the AM ester, the mild detergent Pluronic F-127 (catalog no. 59004) is often used as a dispersing agent to facilitate cell loading. Fura-2, AM ester itself does not bind Ca2+, but it is readily hydrolyzed to Fura-2 by endogenous esterases once it enters cells.
Fura-2 fluorescence can be detected using flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Upon calcium binding, the fluorescent excitation maximum of the indicator undergoes a blue shift from 363 nm (Ca2+-free) to 335 nm (Ca2+-saturated), while the fluorescence emission maximum is relatively unchanged at ~510 nm. A major use of Fura-2 is in drug discovery, for measuring intracellular calcium mobilization after activation of G protein-coupled receptors (GPCRs) and calcium channels.
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Formula:
C₄₄H₄₇N₃O₂₄ MW: 1001.857 g/mol Storage Temperature: Freezer |
MDL Number:
MFCD00036976 CAS Number: 108964-32-5 |
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