FLUO 3/AM 95 HPLC_ASSAY_METHOD fluorescent indicator, Calcium determination

Supplier: BIOTIUM, INC

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FLUO 3/AM 95 HPLC_ASSAY_METHOD fluorescent indicator, Calcium determination

FLUO 3/AM

Membrane-permeant AM ester form of Fluo-3 that can be loaded into cells via incubation. Fluo-3 AM ester itself does not bind Ca2 , but it is readily hydrolyzed to Fluo-3 by endogenous esterases once the dye is inside the cells. An important application of Fluo-3 AM ester is its use in high throughput drug screening.

Abs/Em (after hydrolysis): 506/526 nm (low or high [Ca2 ] )
Extinction Coefficient (after hydrolysis): 86,000 M-1 cm-1 (506 nm)
Kd (Ca2 ) (after hydrolysis): 450 nM
Cell permeability: Membrane permeant
Orange red solid soluble in DMSO
Store -20°C and protect from light, especially when in solution

Because of the relatively low water solubility of the AM ester, Pluronic F-127, a mild detergent, is often used as a dispersing agent to facilitate dye loading. See the product information sheet for more information.

Formula: C₅₁H₅₀Cl₂N₂O₂₃
MW: 1129.85 g/mol
Storage Temperature: Freezer

CAS Number: 121714-22-5

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