Cell Lysis Buffer, Rockland Immunochemicals

Supplier: Bandelin Electronic GmbH &Co.KG

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MB-078-0015 MB-078-0050

76236-538EA 67.91 USD

76236-538 76236-540

Cell Lysis Buffer, Rockland Immunochemicals

Buffers Extraction Buffers

Cell Lysis Buffer is used to lyse cells under non-denaturing conditions.

Ready to use for all types of human or animal cells
Lyse cells under non-denaturing conditions
Protease inhibitors may be recommended, not included in product composition

If recommended add 1 mM PMSF or other protease inhibitor immediately before use. Keep all reagents and lysates in ice.

For adherent cells add 400µL of buffer per 10cm flask once they have been washed in 1X PBS.
For suspended cells add 400 µL of buffer per million cells once they have been washed in 1X PBS and pelleted.
For tissue lysates add 20µL of buffer per 1mg of tissue followed by homogenization and sonication.

Application Note: The buffer is ready to use for all types of human or animal cells. Lysed cells can be used for Western Blot, co-Immunoprecipitation, Chromatin IP (ChIP), enzyme activity assays, and various other cell based assays.

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