Bovine Ribonuclease A (from Pancreas), MP Biomedicals

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76204-274EA 61.98 USD
76204-274 76204-276 76204-278 76204-280 76204-282
Bovine Ribonuclease A (from Pancreas), MP Biomedicals
Enzymes

Ribonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG.


  • Grade : Molecular Biology Reagent
  • Specific Activity : ≥70 Kunitz units/mg
  • Key Applications : Purification of DNA; Analysis of RNA sequences; RNase protection assays; Hydrolyze RNA contained in protein samplesApplication Areas
  • Product Type : Proteins, Enzymes & Peptides
  • Extinction Coefficient : (E1%) E1%280 = ~7.3
  • Presentation : White Powder
  • Format : Lyophilized powder, Salt-free and protease-free
  • Isoelectric point (pI) : 9.45
  • pH Optimum pH: 7.0 to 7.5
  • NOTES : Inhibitors: Ribonuclease is inhibited by heavy metal ions and is competitively inhibited by DNA. The effect of denatured DNA is much greater than that of the native nucleic acid. Composition: The composition and structure of ribonuclease A has been extensively investigated. The tertiary structure indicates that flexibility in a three-dimensional structure is essential for optimal catalytic activity.
  • Specificity : The enzyme is specific for pyrimidine nucleoside linkages, i.e., those cleaving cyclic 2',3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains.
  • Solubility: Dissolves readily at 2 mg/ml in analytical grade water to give a clear colorless solution.

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