Bovine Ribonuclease A (from Pancreas), MP Biomedicals
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0219398010
0219398050
0219398083
0219398090
0219398091
76204-274EA
61.98
USD
76204-274
76204-276
76204-278
76204-280
76204-282
Bovine Ribonuclease A (from Pancreas), MP Biomedicals
Enzymes
Ribonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG.
- Grade : Molecular Biology Reagent
- Specific Activity : ≥70 Kunitz units/mg
- Key Applications : Purification of DNA; Analysis of RNA sequences; RNase protection assays; Hydrolyze RNA contained in protein samplesApplication Areas
- Product Type : Proteins, Enzymes & Peptides
- Extinction Coefficient : (E1%) E1%280 = ~7.3
- Presentation : White Powder
- Format : Lyophilized powder, Salt-free and protease-free
- Isoelectric point (pI) : 9.45
- pH Optimum pH: 7.0 to 7.5
- NOTES : Inhibitors: Ribonuclease is inhibited by heavy metal ions and is competitively inhibited by DNA. The effect of denatured DNA is much greater than that of the native nucleic acid. Composition: The composition and structure of ribonuclease A has been extensively investigated. The tertiary structure indicates that flexibility in a three-dimensional structure is essential for optimal catalytic activity.
- Specificity : The enzyme is specific for pyrimidine nucleoside linkages, i.e., those cleaving cyclic 2',3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains.
- Solubility: Dissolves readily at 2 mg/ml in analytical grade water to give a clear colorless solution.
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