Avidin (from Egg white), MP Biomedicals
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Avidin is a basic glycoprotein isolated from raw egg white. It exhibits high binding affinity for biotin and is capable of producing biotin deficiency in rats and chicks. It also occurs in the white portion of eggs and the oviducts of birds and amphibia. It is destroyed by cooking or irradiation.
- Ready for CE IVD labeling of clinical applications
- Specific activity: 10 to 15 u/mg solid
- Solubility: Soluble in water or dilute aqueous buffer
- Unit definition: One unit will bind 1 µg of D-biotin at pH 8.9
Avidin is useful in a biotin-avidin system for labeling biomolecules for receptor studies, immunoassays and immunohistological methods. Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol. Egg white avidin has been used as a cross-linker for biotin-conjugated antibodies. Avidin also been used as a blocking agent in ELISA applications.
Caution: Research use only (RUO).
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