M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant, Promega

Supplier: EBRO Electronic GmbH

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M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant, Promega

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant, Promega

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant, Promega

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant, Promega

M3681 M3682 M3683

PAM3681EA 94.75 USD

PAM3681 PAM3682 PAM3683

M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant, Promega

Nucleic Acid Reagents RT-PCR and cDNA Reagents

Point mutation of M-MLV RT resulting in no RNase H activity. Suitable for rare or long transcripts.

High Yields of cDNA from Long RNA Templates (>5kb)

Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (M-MLV RT [H-]), Point Mutant, is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long RNA templates (>5kb). The lack of RNase H activity is beneficial for this application, as RNase H can start to degrade templates when incubation times are long, as they may be when synthesizing long cDNAs. Although many researchers are successful in using M-MLV RT (H+) for analytical and some preparative cDNA applications, reverse transcriptases lacking RNase H activity provide another option to prepare long cDNAs and libraries containing a high percentage of full-length cDNA.

Caution: Storage temperature -30°C to -10°C

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