β-(N-Morpholino)ethanesufonic acid (MES) monohydrate ≥99%, white crystalline powder for molecular biology
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MES is a zwitterionic buffer. One of the "Good" buffers developed for biological applications. It has the advantages of maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pK with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range.
A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa ± 1). Alternatively, volumes of equimolar MES free acid and sodium or potassium MES can be mixed to attain the desired pH.
- Molecular Biology Reagent Grade
- Presentation: White Crystalline Powder
- Metal Binding Constants (log K) at 20 °C for 0.1 M Solution: Mg2+: 0.8; Ca2+: 0.7; Mn2+: 0.7; Cu2+: negligible
- Sterilization should be done by filtration through a 0.2 micron filter. Autoclaving is not recommended. Autoclaving yields an unidentified yellow by-product.
Formula:
C₆H₁₃NO₄S·1H₂O |
MDL Number:
MFCD00149409 CAS Number: 145224-94-8 |
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