β-(N-Morpholino)ethanesufonic acid (MES) monohydrate ≥99%, white crystalline powder for molecular biology
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Synonyms:
MES monohydrate, 2-(N-Morpholino)ethanesulphonic acid monohydrate, 2-Morpholinoethanesulphonic acid hydrate, 2-Morpholinoethanesulfonic acid hydrate, 2-(N-Morpholino)ethanesulfonic acid monohydrate
MES is a zwitterionic buffer. One of the "Good" buffers developed for biological applications. It has the advantages of maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pK with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range.
A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa ± 1). Alternatively, volumes of equimolar MES free acid and sodium or potassium MES can be mixed to attain the desired pH.
- Molecular Biology Reagent Grade
- Presentation: White Crystalline Powder
- Metal Binding Constants (log K) at 20 °C for 0.1 M Solution: Mg2+: 0.8; Ca2+: 0.7; Mn2+: 0.7; Cu2+: negligible
- Sterilization should be done by filtration through a 0.2 micron filter. Autoclaving is not recommended. Autoclaving yields an unidentified yellow by-product.
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Formula:
C₆H₁₃NO₄S·1H₂O MW: 213.25 g/mol Melting Pt: >300 °C (lit.) Storage Temperature: Ambient |
MDL Number:
MFCD00149409 CAS Number: 145224-94-8 |
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Specification Test Results
| Identity Test | Passes |
| Purity | ≥99% |
| pH (1% aq soln) | 2.5-4.0 |
| Loss on Drying | <10% |
| OD 260 (5% aq soln) | ≤0.3 |
| Water Content | 8.0-8.9% |
| Infared | Complies |
| Solubility 0.1M water | Clear, Colorless Solution |
| Residue on Ignition | ≤0.1% |
| pKa 25°C | 5.9-6.3 |
| Heavy Metals | ≤5ppm |
| Chloride | ≤0.01% |
| Sulfate | ≤0.05% |
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