Cyclic AMP ELISA Kit, Cayman Chemical Company

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581001-480 581001-96
75817-362EA 1645.15 USD
75817-362 75817-364
Cyclic AMP ELISA Kit, Cayman Chemical Company
Assays ELISAs

For direct quantification of cAMP from a variety of biological samples


  • Measure acetylated or non-acetylated cAMP in cell lysates, tissue homogenates, plasma or urine
  • Assay 24 samples in triplicate or 36 samples in duplicate
  • Measure cAMP levels down to 0.1 pmol/ml
  • Incubation: 18 hours | Development: 60-90 minutes | Read: Colorimetric at 405-420 nm
  • Contains acetic anhydride


Cyclic AMP (cAMP) is is a ubiquitous cellular second messenger that is a critical component of a signal transduction pathway linking membrane receptors and their ligands to the activation of internal cellular enzymatic activity and gene expression. cAMP is synthesized from ATP by membrane-bound adenylate cyclase. Binding of certain ligands or hormones to their specific G-protein couple receptors activates GTP binding proteins (Gs or Gi) which either stimulate or inhibit adenylate cyclase. cAMP activates or inhibits various enzymes or cascade of enzymes by promoting their phosphorylation or dephosphorylation. The cAMP signal is neutralized by hydrolysis of cAMP to AMP by phosphodiesterases. Therefore, the concentration of cAMP in a cell is a function of the ratio of the rate of synthesis from ATP by adenylate cyclase and its rate of breakdown to AMP by specific phosphodiesterases.


Kit contains: Antiserum, Tracer, Standard, Buffers, Reagents, and Plate

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