Chloroform ≥99.8% ACS
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Chloroform is typically used as a solvent and as a cleansing agent. Procedures have been described for the use of chloroform in lambda plaques storage, lambda cDNA storage, the removal of mineral oil from PCR reaction samples, oligonucleotide purification, a hydroxyl radical footprinting protocol, a transcriptional run-on assay protocol, and an overlay assay for beta-galactosidase activity. It has also been used with phenol in such procedures as DNA recovery from polyacrylamide gels, ethidium bromide removal from DNA preparations, lysis protocols for plasmid DNA isolation, RNase removal, and purification of yeast DNA. A protocol describes the use of chloroform in a high-performance thin-layer chromatography protocol for sphingomyelin analysis. Chloroform can also be used in chloroform/methanol mixtures for the isolation of cardiolipids from Geobacillus stearothermophilus and their subsequent MS analysis. The isolation of the bacteriocin amylovorin L471 from Lactobacillus amylovorus DCE 471 in culture broth has been reported, using chloroform/methanol extraction and precipitation in the procedure. Chloroform/2-butanol mixtures can be used for the extraction of steroid sulfates for analysis by nanoelectrospray ionization mass spectrometry.
Formula:
CHCl₃ MW: 119.38 g/mol Boiling Pt: 61.7 (lit.) Melting Pt: -63 (lit.) Density: 1.476…1.478 g/cm³ (20 °C) (lit.) Storage Temperature: Ambient |
MDL Number:
MFCD00000826 CAS Number: 67-66-3 EINECS: 200-663-8 UN: 1888 ADR: 6.1 III Merck Index: 13,02160 |
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Specification Test Results
Physical Appearance | Liquid |
Auto Ignition | Above 1000 °C(Lit.) |
Vapor Density | 4.36 (air = 1)(Lit.) |
Vapor Pressure | 211 hPa at 20 °C(Lit.) |
Refractive Index | n20/D 1.445(lit.) |
UV/Visible Absorbance | λ: 245 nm Amax: 1.00, λ: 255 nm Amax: 0.25, λ: 260 nm Amax: 0.15, λ: 270 nm Amax: 0.05, λ: 290-400 nm Amax: 0.01(Lit.) |
Color APHA | ≤10 |
Solubility | Soluble in water (1 mL/200 mL water); miscible with ethanol, benzene, ether, petroleum ether, carbon tetrachloride, carbon disulfide, oils. |
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