Pierce™ EZ-Link® Sulfhydryl Reactive Biotinylation Reagents, Thermo Scientific
Supplier: Paul Schöndorf Metallwaren GmbHTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptides. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.
Maleimide-PEG2 and PEG11-Biotin: Water-soluble
Biotin-BMCC: Reacts with sulfhydryl (-SH) groups at 6.5 to 7.5 pH. Avoids potential modification of tyrosine residues that can be associated with Iodoacetyl-Biotin. Must be dissolved in DMSO or DMF before use.
Iodoacetyl-PEG2-Biotin: Water soluble. Reacts with sulfhydryl (-SH) groups at basic to neutral pH. Coupling reactions occur in the dark.
Iodoacetyl-LC-Biotin: Reacts with sulfhydryl (-SH) groups at basic to neutral pH. Coupling reactions occur in the dark. Must be dissolved in DMSO or DMF before use.
Reacts with sulfhydryl (-SH) groups at acidic to neutral pH. PEG11-Biotin has an extra long spacer arm that reduces steric hindrance.
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