Trypsin/Lys-C Mix, MS Grade, Promega

Supplier: EBRO Electronic GmbH
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Trypsin/Lys-C Mix, MS Grade, Promega
Protein Extraction Protein Extraction Reagents

Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution.


  • Improve Protein Digestion with this Mix of Trypsin Gold and rLys-C
  • Use standard overnight digestion, nondenaturing conditions
  • Increased peptide recovery, enhanced protein quantitation, improved reproducibility
  • Tolerant to trypsin-inhibiting contaminants


Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution. Using the conventional trypsin digestion protocol (i.e., overnight incubation at nondenaturing conditions), Trypsin/Lys-C Mix improves protein digestion by eliminating the majority of missed cleavages, which occur at prominent quantities in trypsin digests. Trypsin/Lys-C Mix enhances digestion and compensates for the trypsin proteolytic inefficiency at lysine sites. Replacing trypsin with Trypsin/Lys-C Mix in this conventional protocol leads to multiple benefits for protein analysis including more accurate mass spectrometry-based protein quantitation and improved protein mass spectrometry analytical reproducibility. Trypsin/Lys-C Mix also provides greater tolerance to trypsin-inhibiting agents, assuring efficient digestion of proteins for which protein purification is limited or not feasible.

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