pHAb Amine Reactive Dyes, Promega
Supplier: EBRO Electronic GmbHTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
pHAb Amine Reactive Dye exhibits very low fluorescence at pH > 7 and much higher fluorescence as the pH of the solution becomes acidic. pHAb Dyes are designed specifically for antibody labeling.
- Measure Antibody Internalization in Real Time
- Compatible with 96-well, plate-based assay
- High signal-to-background ratios
- On-Bead conjugation
pHAb Amine Dye is a pH sensor dye that have very low fluorescence at pH > 7 and a dramatic increase in fluorescence as the pH of the solution becomes acidic. pHAb Dyes have excitation maxima (Ex) at 532nm and emission maxima (Em) at 560nm. pHAb Dyes are designed specifically for antibody labeling. pHAb Amine Reactive Dye has a succinimidyl ester group that reacts with primary amines available on the lysine amino acids on the antibodies. A key feature of pHAb Amine Reactive Dye is that it has two sulfonate groups per dye, which increases solubility and reduces the aggregation often seen with other non-sulfonated dyes. pHAb Amine Reactive Dye maintains their fluorescence response to pH change even after antibody conjugation. Even though antibody conjugation is the key application, any protein containing primary amines on lysine amino acids can be conjugated with pHAb Amine Reactive Dye.
A key feature of pHAb Dyes is the two sulfonate groups per dye, which increase solubility and reduce the aggregation often seen with other non-sulfonated dyes. pHAb Dyes maintain their fluorescence response to pH change even after antibody conjugation. Even though antibody conjugation is the key application, any protein containing primary amines on lysine amino acids or thiols on cysteine amino acids can be conjugated with pHAb Dyes.
pHAb Dyes are designed specifically for antibody labeling and are available in two reactive forms suitable for antibody conjugations. pHAb Amine Reactive Dye has a succinimidyl ester group that reacts with primary amines available on the lysine amino acids on the antibodies. pHAb Thiol Reactive Dye has a maleimide group that reacts with thiols. This maleimide group is conjugated to the antibody after a reducing agent reduces the cysteine disulfide bonds in the hinge region of the antibody.
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