Recombinant Terminal Deoxynucleotidyl Transferase

Supplier: EBRO Electronic GmbH

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Recombinant Terminal Deoxynucleotidyl Transferase

Recombinant Terminal Deoxynucleotidyl Transferase

Recombinant Terminal Deoxynucleotidyl Transferase

Recombinant Terminal Deoxynucleotidyl Transferase

M1871 M1875

PAM1871EA 131.05 USD

PAM1871 PAM1875

Recombinant Terminal Deoxynucleotidyl Transferase

Enzymes

TdT catalyzes the addition of mononucleotides to the terminal 3'-OH of a DNA initiator accompanied by the release of inorganic phosphate.

For labeling in vitro, and cDNA cloning
Adds Mononucleotides to the Terminal 3´-OH of ssDNA and Releases PPi
Adds mononucleotide tails to any type of 3´ end (3´ and 5´ overhangs or blunt ends) due to the presence of 1mM CoCl2 in the reaction buffer
Quality tested for labeling of apoptotic DNA ends using the procedure outlined in the DeadEnd™ Fluorometric TUNEL System Technical Bulletin #TB235 for each lot of enzyme
Supplied at a concentration of 30u/microl

Terminal Deoxynucleotidyl Transferase, Recombinant, catalyzes the repetitive addition of mononucleotides to the terminal 3'-OH of a DNA initiator accompanied by the release of inorganic phosphate. Single-stranded DNA is preferred as an initiator. Polymerization is not template-dependent. The addition of 1mM Co(2+) (as CoCl(2)) in the reaction buffer allows the tailing of 3'-ends with varying degrees of efficiency.

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