Pierce™ Recombinant Protein A
Supplier: Paul Schöndorf Metallwaren GmbHTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
Purified (unconjugated) Thermo Scientific Pierce Recombinant Protein A is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.
- Contains four Fc-binding domains per protein
- Better than Protein G for polyclonal IgG from rabbit, pig, dog and cat serum
- Poorer than Protein G for mouse IgG1, human IgG3 and rat, goat and cow antibodies
Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E. coli and functions essentially the same as native Protein A. Contains four high-affinity binding sites capable of interacting with the Fc region from IgG of several species including human and rabbit. Optimal binding occurs at pH 8.2, although binding is also effective at neutral or physiological conditions (pH 7.0 to 7.6)
Properties of Recombinant Protein A:
Source: E. coli
Molecular Weight:
44,600 (Apparent MW by SDS-PAGE: 45,000)
Form: salt-free powder
A280 of 0.1% solution:
0.165
Isoelectric point (pI): 4.7 to 4.8
Caution: For Research Use Only. Not for use in diagnostic procedures.
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