Pierce™ Recombinant Protein A

Supplier: Paul Schöndorf Metallwaren GmbH
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21184 77673 77674
PI21184EA 140.68 USD
PI21184 PI77673 PI77674
Pierce™ Recombinant Protein A
Proteins and Peptides

Purified (unconjugated) Thermo Scientific Pierce Recombinant Protein A is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.


  • Contains four Fc-binding domains per protein
  • Better than Protein G for polyclonal IgG from rabbit, pig, dog and cat serum
  • Poorer than Protein G for mouse IgG1, human IgG3 and rat, goat and cow antibodies


Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E. coli and functions essentially the same as native Protein A. Contains four high-affinity binding sites capable of interacting with the Fc region from IgG of several species including human and rabbit. Optimal binding occurs at pH 8.2, although binding is also effective at neutral or physiological conditions (pH 7.0 to 7.6)


Properties of Recombinant Protein A:
Source: E. coli
Molecular Weight:


44,600 (Apparent MW by SDS-PAGE: 45,000)
Form: salt-free powder
A280 of 0.1% solution:


0.165
Isoelectric point (pI): 4.7 to 4.8


Caution: For Research Use Only. Not for use in diagnostic procedures.

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