Ghost Dye™ UV 450 for flow cytometry
Supplier: CYTEK BIOSCIENCES, INCTotal Ratings: 0
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Ghost Dye™ UV 450 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications
- Excitation Laser UV (355 nm)
- Emission (nm) 450
- Formulation 1 µL/test in DMSO
This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost UV 450 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.
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