PIPES Free_acid, powder_white
Supplier: TestTotal Ratings: 0
Avg. Ratings: 0.0 out of 5
PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer. Buffers can be made by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the sesquisodium salt and the disodium salt or dipotassium salt, titrating to the appropriate pH.
PIPES is used to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue tissue samples. This will help prevent the loss of lipids. It is used to buffer glutaraldehyde and formaldehyde when fixing fungal zoospores for fluorescence microscopy and electron microscopy.
Zwitterionic Buffer ,Effective buffering range: 6.1 - 7.5 (Merck Index reports 6.0 to 8.5 ), ΔpK/ΔT: 0.0085 ,pKa: 6.8 at 25 °C ; 6.66 at 37 °C
PIPES free acid is not very water soluble (approximately 1 g/L at 100 °C) but will solubilize in 0.5 M NaOH; PIPES salts are water soluble (usually resulting in clear, colorless solutions).
Zwitterionic Buffer ,Effective buffering range: 6.1 - 7.5 (Merck Index reports 6.0 to 8.5 ), ΔpK/ΔT: 0.0085 ,pKa: 6.8 at 25 °C ; 6.66 at 37 °C
PIPES is used to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue tissue samples. This will help prevent the loss of lipids. It is used to buffer glutaraldehyde and formaldehyde when fixing fungal zoospores for fluorescence microscopy and electron microscopy.
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Formula:
C₈H₁₈N₂O₆S₂ MW: 302.37 g/mol Boiling Pt: 499 Melting Pt: 300 Storage Temperature: Ambient |
MDL Number:
MFCD00006159 CAS Number: 5625-37-6 Merck Index: 12,07633 |
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Specification Test Results
| Identity Test | Passes |
| FTIR | Conforms to Standard |
| UV/Vis: OD260 (0.1 M in 0.2 M NaOH) | ≤0.05 |
| UV/Vis: OD280 (0.1 M in 0.2 M NaOH) | ≤0.05 |
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