Guanidinium hydrochloride, white powder, Practical grade
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Synonyms:
Guanadine hydrochloride, Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidine HCI, Guanidine chloride, Guanidinium chloride, Guanidine hydrochloride
Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins.
This strong denaturant can solubilise insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. It is also used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase.
Highly concentrated (6 - 8 M) Guanidine HCl solutions are used to denature native globular proteins.
Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. It is also used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase.
Formula:
NH₂C(=NH)NH₂·HCl MW: 95.53 g/mol |
MDL Number:
MFCD00013026 CAS Number: 50-01-1 |
Specification Test Results
Identity Test | Passes |
Melting Point | 175 - 190 °C |
FTIR | Conforms to Standard |
Loss on Drying | ≤1,0% |
Solubility (6M aq soln) R | Relatively Clear to Cloudy Yellow Solution with Sediment on Standing |
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