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02100878.5 0210087801 0210087805 0210087810 0210087850
IC100878.5EA 44.35 USD
IC100878.5 IC10087801 IC10087805 IC10087810 IC10087850
, powder
4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP)

p nitrophenol is the substrate of choice for use with alkaline phosphatase in ELISA procedures. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 M NaOH and read at 405 nm.


p-nitrophenol is suitable for use as a substrate for alkaline and acid phosphatase.


Typically a pNPP concentration of 1 mg/ml is used


Soluble in water.


Free p-Nitrophenol 0.005%


p nitrophenol is the substrate of choice for use with alkaline phosphatase in ELISA procedures. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 M NaOH and read at 405 nm.

Formula: C₆H₄NNa₂O₆P·6H₂O
MW: 371.144 g/mol
Melting Pt: 300 (lit.)
Storage Temperature: Freezer
MDL Number: MFCD00066288
CAS Number: 333338-18-4

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Specification Test Results

Identity Test Passes
Water Content 27 - 31%
Free p-Nitrophenol ≤0,005%


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