AccuStart™ II Taq DNA Polymerase, QuantaBio
Supplier: POLY SERVICE BVTotal Ratings: 0
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AccuStart™ II Taq DNA Polymerase is a high purity, recombinant Taq DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step
- High-yielding, ultrapure modified Taq DNA polymerase delivers robust, reliable assay sensitivity
- Stringent, ultrapure antibody hotstart enables precise target amplification and ambient room temperature setup
Activated AccuStart II Taq DNA Polymerase possesses 5' ̶ ˃3’ DNA polymerase activity and a double-strand specific 5’ ̶ ˃3’ exonuclease. The polymerase does not have 3’-exonuclease activity and is free of any contaminating endo or exonuclease activities. One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. AccuStart™ II Taq DNA polymerase contains extremely low levels of residual host, E. coli genomic DNA.
AccuStart II Taq DNA polymerase is stable for 2 years when stored in a constant temperature freezer at -20°C.
Upon heat activation (1 minute at 94°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimized buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.
Ordering information: AccuStart II Taq DNA Polymerase includes 5 units/?L in 50% glycerol, 20 mM Tris-HCl, 40 mM NaCl, 0.1 mM EDTA, and stabilizers, 10X PCR Buffer II Optimized 10X-concentrated buffer and 50 mM MgCl2.
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