Citifluor™ Antifadent Mountant Solutions, Electron Microscopy Sciences
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Citifluor™ mountant media containing antifadents solutions reduce the photo-bleaching or fading of the fluorescence of dyes used for labeling biological species.
- For labeling biological species
- None of the antifadents is based on p-phenylenediamine
- All solutions may be stored at room temperature
Glycerol Based non-harding mountants: AF1 A glycerol-PBS solution contains an amine antifadent. AF1 plus DAPI contains both an amine antifadent and the DNA stain, DAPI. AF2 A glycerol solution contains an amine antifadent that allows you to choose your own buffer solution. AF4 A glycerol solution of n-propyl gallate. Glycerol Free formulations. AF3 is a PBS solution containing an amine-based antifadent. CFPVOH is an aqueous solution of poly (vinyl alcohol). Provided care is taken not to allow the water to evaporate, it will remain fluid. Retardation of photobleaching is affected by the addition of the aqueous antifadent solution AF100. AF100 Is a PBS solution of an amine-based antifadent that if used as an additive to CFPVOH (1-part AF100 to 9 parts CFPVOH). Provided care is taken not to allow the water to evaporate, it will remain fluid. AFR3 is a PBS solution containing a NEW non-amine, non-phenolic antifadent.
High Refractive Index Glycerol based solutions: These solutions have a refractive index that matches that of the glass of the coverslip thereby minimising the effects of spherical aberration. Such mountants are invaluable for imaging the internal structures of samples by techniques such as single multi-photon confocal. There are four high refractive glycerol-based solutions. CFM-1 is a glycerol-PBS buffered solution having a refractive index of ~1.52 which can be used for transmission microscopy as well as with epifluorescence microscopy. CFM-1 plus AF is a glycerol-based solution having a refractive index of ~1.52 (at room temperature) that contains an amine antifadent. CFM-2 is a glycerol-tris amine buffered solution having a refractive index of ~1.52 (at room temperature) and a pH of ~8.5. CFM-3 – This glycerol-based antifadent contains a phenolic antifadent of neutral pH and a refractive index of ~1.52 which also acts as a Clearing Solution enabling visualisation of fluorochromes deep within the sample. CFMR2 – This product was designed for use with samples labeled with GFP. It contains a unique antifadent which does not de-oxygenate the solution which is an essential property if the GFP is not to bleach. High Refractive Index Glycerol Free Solutions: AF87 is an immersion oil having a refractive index of 1.52 and contains an antifadent. It may be used as an immersion oil and as a mountant. Since AF87 is an oil that is immiscible with water, it is essential that specimens are dehydrated before application of the mountant.
Hardening mountants are aqueous solutions containing a polymer such a poly (vinyl alcohol) (PVOH). Glycerol Based are available in two formulations. AF200 is a glycerol solution containing an amine-based antifadent. AF300 is a glycerol solution containing a phenolic type of antifadent.
Film Forming Polymer Solutions: Tris-MWL 4-88 is a classical, popular mountant solution based on Mowiol® 4-88, glycerol, water and tris-amine buffer. Following evaporation of the water a film of weak to medium strength is formed. To have effective reduction in photobleaching, it should be used with either AF100, AF200 or AF300. CFPVOH is an aqueous solution of poly (vinyl alcohol) and is designed to be used with the glycerol-based antifadent solutions AF200 or AF300. Following evaporation of the water films of medium hardness are produced. Permafix PF is an aqueous solution of a chemically modified PVOH which may be used with AF200 or AF300 to give solutions which following loss of water by evaporation give films of medium to high hardness.
Glycerol Free: PVP plus antifadent is an aqueous solution of poly (vinyl pyrrolidone) containing an amine-based antifadent. The solutions are stable over long periods e.g. more than five years. CFPVOH plus antifadent is an aqueous solution of a carefully selected PVOH containing an amine-based antifadent and has a shelf life 6 month. High Refractive Index Glycerol Free Solution: Permafix PF/HRF this is an aqueous solution of a chemical.
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